ArticlePlant physiology2010
Nitric oxide functions as a signal and acts upstream of AtCaM3 in thermotolerance in Arabidopsis seedlings.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 158 citations in OpenAlex.
- Nitrate Reductase GenesBiomolecules · 2026Article
- Transcriptomic analysis of reduced thermotolerance inFrontiers in plant science · 2026Article
- Zea Maize Calmodulin (Plants (Basel, Switzerland) · 2025Article
- The Central Role of GSNOR: Decoding Nitric Oxide Signaling for Crop Stress Tolerance.International journal of molecular sciences · 2025Review
- The Hidden Architects: Nitric Oxide and Redox Dynamics in Plant Stem Cell Homeostasis.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Nitric oxide-mediated thermomemory: a new perspective on plant heat stress resilience.Frontiers in plant science · 2025Review
- Functional validation of AaCaM3 response to high temperature stress in Amorphophallus albus.BMC plant biology · 2024Article
- Appraisal of the Role of Gaseous Signaling Molecules in Thermo-Tolerance Mechanisms in Plants.Plants (Basel, Switzerland) · 2024Review
- Calcium Signaling and the Response to Heat Shock in Crop Plants.International journal of molecular sciences · 2023Review
- Light signaling as cellular integrator of multiple environmental cues in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- Transcriptome Analysis Reveals the Dynamic and Rapid Transcriptional Reprogramming Involved in Heat Stress and Identification of Heat Response Genes in Rice.International journal of molecular sciences · 2023Article
- Multiple Ways of Nitric Oxide Production in Plants and Its Functional Activity under Abiotic Stress Conditions.International journal of molecular sciences · 2023Review
- Redox Signaling in Plant Heat Stress Response.Antioxidants (Basel, Switzerland) · 2023Review
- Comparative transcriptome analysis of the mechanism difference in heat stress response betweenFrontiers in genetics · 2023Article
- Functions of nitric oxide-mediated post-translational modifications under abiotic stress.Frontiers in plant science · 2023Review
- Brassinosteroids promote thermotolerance through releasing BIN2-mediated phosphorylation and suppression of HsfA1 transcription factors in Arabidopsis.Plant communications · 2022Article
- Gene Networks Involved in Plant Heat Stress Response and Tolerance.International journal of molecular sciences · 2022Review
- Hydrogen Sulfide, Ethylene, and Nitric Oxide Regulate Redox Homeostasis and Protect Photosynthetic Metabolism under High Temperature Stress in Rice Plants.Antioxidants (Basel, Switzerland) · 2022Article
- The calcium signaling module CaM-IQM destabilizes IAA-ARF interaction to regulate callus and lateral root formation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Reproductive-Stage Heat Stress in Cereals: Impact, Plant Responses and Strategies for Tolerance Improvement.International journal of molecular sciences · 2022Review
4 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To characterize the role of nitric oxide (NO) in the tolerance of Arabidopsis (Arabidopsis thaliana) to heat shock (HS), we investigated the effects of heat on three types of Arabidopsis seedlings: wild type, noa1(rif1) (for nitric oxide associated1/resistant to inhibition by fosmidomycin1) and nia1nia2 (for nitrate reductase [NR]-defective double mutant), which both exhibit reduced endogenous NO levels, and a rescued line of noa1(rif1). After HS treatment, the survival ratios of the mutant seedlings were lower than those of wild type; however, they were partially restored in the rescued line. Treatment of the seedlings with sodium nitroprusside or S-nitroso-N-acetylpenicillamine revealed that internal NO affects heat sensitivity in a concentration-dependent manner. Calmodulin 3 (CaM3) is a key component of HS signaling in Arabidopsis. Real-time reverse transcription-polymerase chain reaction analysis after HS treatment revealed that the AtCaM3 mRNA level was regulated by the internal NO level. Sodium nitroprusside enhanced the survival of the wild-type and noa1(rif1) seedlings; however, no obvious effects were observed for cam3 single or cam3noa1(rif1) double mutant seedlings, suggesting that AtCaM3 is involved in NO signal transduction as a downstream factor. This point was verified by phenotypic analysis and thermotolerance testing using seedlings of three AtCaM3-overexpressing transgenic lines in an noa1(rif1) background. Electrophoretic mobility-shift and western-blot analyses demonstrated that after HS treatment, NO stimulated the DNA-binding activity of HS transcription factors and the accumulation of heat shock protein 18.2 (HSP18.2) through AtCaM3. These data indicate that NO functions in signaling and acts upstream of AtCaM3 in thermotolerance, which is dependent on increased HS transcription factor DNA-binding activity and HSP accumulation.
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