ArticleHuman reproduction (Oxford, England)2010
Effect of the method of conception and embryo transfer procedure on mid-gestation placenta and fetal development in an IVF mouse model.
Article in Human reproduction (Oxford, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
52 citing papers in PubMed, 114 citations in OpenAlex.
- Placental development during early pregnancy in sheep: effects of embryo origin on fetal and placental growth and global methylation.Theriogenology · 2013Trial
- Mouse placentae generated by in vitro fertilization exhibit altered gene expression, activated hypoxia responses, and reduced fitness†.Biology of reproduction · 2026Article
- When less really is more: reducing embryo manipulation to lower metabolic stress and improving long term outcomes.Reproduction, fertility, and development · 2026Review
- Reappraisal of adverse obstetric outcomes independently associated with contemporary fertility treatment practices: a US prospective cohort study.American journal of obstetrics and gynecology · 2026Article
- Association between ovarian hyperstimulation syndrome and adverse obstetric and neonatal outcomes in Korea: a nationwide health insurance database study.Journal of assisted reproduction and genetics · 2025Article
- Role of In Vitro Fertilization (IVF) in Unexplained Infertility Management: A Systematic Review.Cureus · 2024Review
- Fertility treatment increases the risk of preterm birth independent of multiple gestations.F&S reports · 2023Article
- Long-Term Effects of ART on the Health of the Offspring.International journal of molecular sciences · 2023Review
- Preconception paternal alcohol exposure decreases IVF embryo survival and pregnancy success rates in a mouse model.Molecular human reproduction · 2023Article
- Paternal epigenetic influences on placental health and their impacts on offspring development and disease.Frontiers in genetics · 2022Review
- Placental Abnormalities are Associated With Specific Windows of Embryo Culture in a Mouse Model.Frontiers in cell and developmental biology · 2022Article
- Article
- Alteration of Genomic Imprinting after Assisted Reproductive Technologies and Long-Term Health.Life (Basel, Switzerland) · 2021Review
- In vitro fertilization is associated with placental accelerated villous maturation.International journal of clinical and experimental pathology · 2021Article
- Effect of Embryo Vitrification on the Steroid Biosynthesis of Liver Tissue in Rabbit Offspring.International journal of molecular sciences · 2020Article
- Sex-specific epigenetic profile of inner cell mass of mice conceived in vivo or by IVF.Molecular human reproduction · 2020Article
- Pre-Implantation Mouse Embryos Cultured In Vitro under Different Oxygen Concentrations Show Altered Ultrastructures.International journal of environmental research and public health · 2020Article
- Increased blood pressure and impaired endothelial function after accelerated growth in IVF/ICSI children.Human reproduction open · 2020Article
- The placental transcriptome of the first-trimester placenta is affected by in vitro fertilization and embryo transfer.Reproductive biology and endocrinology : RB&E · 2019Article
- Animal and Human Tissue Models of Vertical Listeria monocytogenes Transmission and Implications for Other Pregnancy-Associated Infections.Infection and immunity · 2018Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundAbnormal placentation is a potential mechanism to explain the increased incidence of low birthweight observed after IVF. This study evaluates, in a mouse model, whether the method of conception and embryo transfer affect placentation and fetal development.
methodsIVF blastocysts (CF1 x B6D2F1/J) were cultured in Whitten's medium (IVF(WM), n = 55) or K modified simplex optimized medium with amino acids (IVF(KAA), n = 56). Embryos were transferred to the uteri of pseudo-pregnant recipients. Two control groups were created: unmanipulated embryos produced by natural mating (in vivo group, n = 64) and embryos produced by natural mating that were flushed from uterus and immediately transferred to pseudo-pregnant recipients (flushed blastocysts, FB group, n = 57). At gestation age 12.5 days, implantation sites were collected and fixed; fetuses and placentas were weighed and their developmental stage (DS) evaluated. Placental areas and vascular volume fractions were calculated; parametric statistics were applied as appropriate.
resultsIVF fetuses showed a modest but significant delay in development compared with FB mice (P < 0.05). In addition, IVF conceptuses were consistently smaller than FB (P < 0.05). Importantly, these differences persisted when analyzing fetuses of similar DS. The placenta/fetus ratio was larger in the IVF group (IVF(WM) 0.95; IVF(KAA) = 0.90) than the FB group (0.72) (P < 0.05 for all comparisons). Gross morphology of the placenta and ratio labyrinth/fetal area were equivalent in the IVF and FB groups, as were percentage of fetal blood vessels, maternal blood spaces and trophoblastic components.
conclusionsIn vitro embryo culture affects fetal and placental development; this could explain the lower birthweight in IVF offspring.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.