ArticleThe Journal of biological chemistry2010
MicroRNA-221 regulates chondrogenic differentiation through promoting proteosomal degradation of slug by targeting Mdm2.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 91 citations in OpenAlex.
- miR-1247 functions by targeting cartilage transcription factor SOX9.The Journal of biological chemistry · 2013Trial
- Comparative Analysis of Dual-miRNA Mediated Stimulation of Nucleus Pulposus and Bone Marrow Derived Stem Cells for Intervertebral Disc Repair.JOR spine · 2026Article
- A miR-activated hydrogel for the delivery of a pro-chondrogenic microRNA-221 inhibitor as a minimally invasive therapeutic approach for articular cartilage repair.Materials today. Bio · 2025Article
- State of the Art: The Immunomodulatory Role of MSCs for Osteoarthritis.International journal of molecular sciences · 2022Review
- Mesenchymal Stem Cell-Derived Exosomes and MicroRNAs in Cartilage Regeneration: Biogenesis, Efficacy, miRNA Enrichment and Delivery.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Engineered-extracellular vesicles as an optimistic tool for microRNA delivery for osteoarthritis treatment.Cellular and molecular life sciences : CMLS · 2021Review
- Review
- The Role of the Microenvironment in Controlling the Fate of Bioprinted Stem Cells.Chemical reviews · 2020Review
- Intra-articular delivery of extracellular vesicles secreted by chondrogenic progenitor cells from MRL/MpJ superhealer mice enhances articular cartilage repair in a mouse injury model.Stem cell research & therapy · 2020Article
- Molecular regulation of Snai2 in development and disease.Journal of cell science · 2019Review
- The Parkinson's disease gene product DJ-1 modulates miR-221 to promote neuronal survival against oxidative stress.Redox biology · 2018Article
- Article
- Gene therapy for chondral and osteochondral regeneration: is the future now?Cellular and molecular life sciences : CMLS · 2018Review
- The Potential of microRNAs for Stem Cell-based Therapy for Degenerative Skeletal Diseases.Current molecular biology reports · 2017Review
- miR-221 attenuates the osteogenic differentiation of human annulus fibrosus cells.The spine journal : official journal of the North American Spine Society · 2016Article
- The Role of miRNAs in Cartilage Homeostasis.Current genomics · 2015Article
- MicroRNA Levels as Prognostic Markers for the Differentiation Potential of Human Mesenchymal Stromal Cell Donors.Stem cells and development · 2015Article
- Does proteasome regulate the level of microRNA-1 in cardiomyocytes? Application to anoxia-reoxygenation.Molecular and cellular biochemistry · 2015Article
- Bone marrow stroma-derived miRNAs as regulators, biomarkers and therapeutic targets of bone metastasis.BoneKEy reports · 2015Review
- Pro-chondrogenic effect of miR-221 and slug depletion in human MSCs.Stem cell reviews and reports · 2014Review
Corrections and comments
- Retraction · 2020-01-10Duplication of/in Image ·
- Retracted
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are small RNAs that fulfill diverse functions by negatively regulating gene expression. Here, we investigated the involvement of miRNAs in the chondrogenic differentiation of chick limb mesenchymal cells and found that the expression of miR-221 increased upon chondrogenic inhibition. Blockade of miR-221 via peanut agglutinin-based antisense oligonucleotides reversed the chondro-inhibitory actions of a JNK inhibitor on the proliferation and migration of chondrogenic progenitors as well as the formation of precartilage condensations. We determined that mdm2 is a relevant target of miR-221 during chondrogenesis. miR-221 was necessary and sufficient to down-regulate Mdm2 expression, and this down-modulation of Mdm2 by miR-221 prevented the degradation of (and consequently up-regulated) the Slug protein, which negatively regulates the proliferation of chondroprogenitors. These results indicate that miR-221 contributes to the regulation of cell proliferation by negatively regulating Mdm2 and thereby inhibiting Slug degradation during the chondrogenesis of chick limb mesenchymal cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.