ArticleBMC plant biology2010
The mitochondrial gene orfH79 plays a critical role in impairing both male gametophyte development and root growth in CMS-Honglian rice.
Article in BMC plant biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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45 citing papers in PubMed, 102 citations in OpenAlex.
- Unraveling Advances in Rice Male Sterility Systems: From Genetic Basis to Hybrid Breeding Innovation.Plants (Basel, Switzerland) · 2026Review
- The molecular evolutionary basis of species formation revisited.Trends in genetics : TIG · 2025Review
- Selection of reference genes for normalization of mitochondrial gene expression by qRT-PCR in different potato tissues and during anther development.BMC plant biology · 2025Article
- The chimeric gene orf610a reduces cotton pollen fertility by impairing the assembly of ATP synthase.Plant biotechnology journal · 2025Article
- Fine mapping ofMolecular breeding : new strategies in plant improvement · 2025Article
- Fertility restorer geneHorticulture research · 2024Article
- Comparative Analysis of the Mitochondrial Genome of Eggplant (International journal of molecular sciences · 2024Article
- Characterization of the CMS genetic regulation through comparative complete mitochondrial genome sequencing in Nicotiana tabacum.The plant genome · 2024Article
- Establishment and Advances of Third-Generation Hybrid Rice Technology: A Review.Rice (New York, N.Y.) · 2023Review
- An epi-allele of SMS causes Sanming dominant genic male sterility in rice.Science China. Life sciences · 2023Article
- Fine mapping and candidate gene analysis of a novel fertility restorer gene for C-type cytoplasmic male sterility in maize (Zea mays L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Two complementary genes in a presence-absence variation contribute to indica-japonica reproductive isolation in rice.Nature communications · 2023Article
- Article
- Investigation of B-atp6-orfH79 distributing in Chinese populations of Oryza rufipogon and analysis of its chimeric structure.BMC plant biology · 2023Article
- Fujian cytoplasmic male sterility and the fertility restorer geneProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Comparison of Mitochondrial Genomes between a Cytoplasmic Male-Sterile Line and Its Restorer Line for Identifying Candidate CMS Genes inInternational journal of molecular sciences · 2022Article
- Molecular basis of cytoplasmic male sterility and fertility restoration in rice.Plant biotechnology (Tokyo, Japan) · 2021Article
- Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (International journal of molecular sciences · 2021Article
- High-generation near-isogenic lines combined with multi-omics to study the mechanism of polima cytoplasmic male sterility.BMC plant biology · 2021Article
- A novel strategy for creating a new system of third-generation hybrid rice technology using a cytoplasmic sterility gene and a genic male-sterile gene.Plant biotechnology journal · 2021Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCytoplasmic male sterility (CMS) has often been associated with abnormal mitochondrial open reading frames. The mitochondrial gene orfH79 is a candidate gene for causing the CMS trait in CMS-Honglian (CMS-HL) rice. However, whether the orfH79 expression can actually induce CMS in rice remains unclear.
resultsWestern blot analysis revealed that the ORFH79 protein is mainly present in mitochondria of CMS-HL rice and is absent in the fertile line. To investigate the function of ORFH79 protein in mitochondria, this gene was fused to a mitochondrial transit peptide sequence and used to transform wild type rice, where its expression induced the gametophytic male sterile phenotype. In addition, excessive accumulation of reactive oxygen species (ROS) in the microspore, a reduced ATP/ADP ratio, decreased mitochondrial membrane potential and a lower respiration rate in the transgenic plants were found to be similar to those in CMS-HL rice. Moreover, retarded growth of primary and lateral roots accompanied by abnormal accumulation of ROS in the root tip was observed in both transgenic rice and CMS-HL rice (YTA).
conclusionThese results suggest that the expression of orfH79 in mitochondria impairs mitochondrial function, which affects the development of both male gametophytes and the roots of CMS-HL rice.
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