ArticlePloS one2010
Proteomics-based systems biology modeling of bovine germinal vesicle stage oocyte and cumulus cell interaction.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- [High-resolution Differential Proteomic Analysis of Mouse Secondary Oocytes and First Polar Bodies].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2025Article
- Maturational competence of equine oocytes is associated with alterations in their 'cumulome'.Molecular human reproduction · 2024Article
- Identification of novel cattle (Bos taurus) genes and biological insights of their function in pre-implantation embryo development.BMC genomics · 2024Article
- Comparative proteomic analysis of seminal plasma exosomes in buffalo with high and low sperm motility.BMC genomics · 2023Article
- Comparative Proteomic Analyses of Poorly Motile Swamp Buffalo Spermatozoa Reveal Low Energy Metabolism and Deficiencies in Motility-Related Proteins.Animals : an open access journal from MDPI · 2022Article
- Dissecting the roles of Haspin and VRK1 in histone H3 phosphorylation during mitosis.Scientific reports · 2022Article
- Genetic and Genomic Analyses of Service Sire Effect on Female Reproductive Traits in Holstein Cattle.Frontiers in genetics · 2021Article
- Oocyte Selection for In Vitro Embryo Production in Bovine Species: Noninvasive Approaches for New Challenges of Oocyte Competence.Animals : an open access journal from MDPI · 2020Review
- Transcriptomic and proteomic analyses of ovarian follicles reveal the role of VLDLR in chicken follicle selection.BMC genomics · 2020Article
- The bovine cumulus proteome is influenced by maturation condition and maturational competence of the oocyte.Scientific reports · 2020Article
- Proteomics Analysis Reveals that Warburg Effect along with Modification in Lipid Metabolism Improves In Vitro Embryo Development under Low Oxygen.International journal of molecular sciences · 2020Article
- Lipid Identification and Transcriptional Analysis of Controlling Enzymes in Bovine Ovarian Follicle.International journal of molecular sciences · 2018Article
- Integrated Analysis of Quantitative Proteome and Transcriptional Profiles Reveals the Dynamic Function of Maternally Expressed Proteins After Parthenogenetic Activation of Buffalo Oocyte.Molecular & cellular proteomics : MCP · 2018Article
- Candidate genes for male and female reproductive traits in Canchim beef cattle.Journal of animal science and biotechnology · 2017Article
- Comparative Proteomic Analysis of Buffalo Oocytes Matured in vitro Using iTRAQ Technique.Scientific reports · 2016Article
- Bioengineering the ovarian follicle microenvironment.Annual review of biomedical engineering · 2014Review
- Systems biology and proteomic analysis of cerebral cavernous malformation.Expert review of proteomics · 2014Article
- Proteomes of animal oocytes: what can we learn for human oocytes in the in vitro fertilization programme?BioMed research international · 2014Review
- Extensive effects of in vitro oocyte maturation on rhesus monkey cumulus cell transcriptome.American journal of physiology. Endocrinology and metabolism · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOocytes are the female gametes which establish the program of life after fertilization. Interactions between oocyte and the surrounding cumulus cells at germinal vesicle (GV) stage are considered essential for proper maturation or 'programming' of oocytes, which is crucial for normal fertilization and embryonic development. However, despite its importance, little is known about the molecular events and pathways involved in this bidirectional communication. METHODOLOGY/PRINCIPAL
findingsWe used differential detergent fractionation multidimensional protein identification technology (DDF-Mud PIT) on bovine GV oocyte and cumulus cells and identified 811 and 1247 proteins in GV oocyte and cumulus cells, respectively; 371 proteins were significantly differentially expressed between each cell type. Systems biology modeling, which included Gene Ontology (GO) and canonical genetic pathway analysis, showed that cumulus cells have higher expression of proteins involved in cell communication, generation of precursor metabolites and energy, as well as transport than GV oocytes. Our data also suggests a hypothesis that oocytes may depend on the presence of cumulus cells to generate specific cellular signals to coordinate their growth and maturation. CONCLUSIONS/SIGNIFICANCE: Systems biology modeling of bovine oocytes and cumulus cells in the context of GO and protein interaction networks identified the signaling pathways associated with the proteins involved in cell-to-cell signaling biological process that may have implications in oocyte competence and maturation. This first comprehensive systems biology modeling of bovine oocytes and cumulus cell proteomes not only provides a foundation for signaling and cell physiology at the GV stage of oocyte development, but are also valuable for comparative studies of other stages of oocyte development at the molecular level.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.