ArticleInvestigative ophthalmology & visual science2010
Reproducibility of spectral-domain optical coherence tomography total retinal thickness measurements in mice.
Article in Investigative ophthalmology & visual science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 53 citations in OpenAlex.
- Automated animal gimbal steering for retinal imaging and stimulation.Biomedical optics express · 2026Article
- Biological Correlations and Confounders for Quantification of Retinal Ganglion Cells by Optical Coherence Tomography Based on Studies of Outbred Mice.Translational vision science & technology · 2022Article
- Aquaporin-4 prevents exaggerated astrocytosis and structural damage in retinal inflammation.Journal of molecular medicine (Berlin, Germany) · 2022Article
- Evaluation of factors related to Anaesthesia-induced Lens opacity in experimental mice.Laboratory animal research · 2020Article
- In Vivo Imaging of the Retina, Choroid, and Optic Nerve Head in Guinea Pigs.Current eye research · 2018Article
- In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy.Journal of visualized experiments : JoVE · 2017Article
- Assessing retinal ganglion cell damage.Eye (London, England) · 2017Review
- Whole-body positional manipulators for ocular imaging of anaesthetised mice and rats: a do-it-yourself guide.BMJ open ophthalmology · 2017Article
- Automated segmentation of mouse OCT volumes (ASiMOV): Validation & clinical study of a light damage model.PloS one · 2017Article
- Quantitative Analysis of Mouse Retinal Layers Using Automated Segmentation of Spectral Domain Optical Coherence Tomography Images.Translational vision science & technology · 2015Article
- Correlation of in vivo and in vitro methods in measuring choroidal vascularization volumes using a subretinal injection induced choroidal neovascularization model.Chinese medical journal · 2015Article
- Nicotine accelerates diabetes-induced retinal changes.Current eye research · 2015Article
- Automated 3D Segmentation of Intraretinal Surfaces in SD-OCT Volumes in Normal and Diabetic Mice.Translational vision science & technology · 2014Article
- Reproducibility of circumpapillary retinal nerve fiber layer measurements using handheld optical coherence tomography in sedated children.American journal of ophthalmology · 2014Article
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- Lateral and axial measurement differences between spectral-domain optical coherence tomography systems.Journal of biomedical optics · 2014Article
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- Functional and morphological analysis of the subretinal injection of human retinal progenitor cells under Cyclosporin A treatment.Molecular vision · 2014Article
- Age-related dystrophic changes in corneal endothelium from DNA repair-deficient mice.Aging cell · 2013Article
- Adaptive optics optical coherence tomography for in vivo mouse retinal imaging.Journal of biomedical optics · 2013Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo test the reproducibility of spectral-domain optical coherence tomography (SD-OCT) total retinal thickness (TRT) measurements in mice.
methodsC57Bl/6 mice were anesthetized, and three repeated volumetric images were acquired in both eyes with SD-OCT (250 A-scans × 250 frames × 1024 samplings), centered on the optic nerve head (ONH). The mice were repositioned between scans. TRT was automatically measured within a sampling band of retinal thickness with radii of 55 to 70 pixels, centered on the ONH by using custom segmentation software. The first volumetric image acquired in a given eye was used to register the remaining two SD-OCT images by manually aligning the en face images with respect to rotation and linear translation. Linear mixed-effects models were fitted to global and quadrant thicknesses, taking into account the clustering between eyes, to assess imprecision (measurement reproducibility).
resultsTwenty-six eyes of 13 adult mice (age 13 weeks) were imaged. The mean global TRT across all eyes was 298.21 μm, with a mouse heterogeneity standard deviation (SD) of 4.88 μm (coefficient of variation [CV] = 0.016), an eye SD of 3.32 μm (CV = 0.011), and a device-related imprecision SD of 2.33 μm (CV = 0.008). The superior quadrant had the thickest mean TRT measurement (310.38 μm) and the highest (worst) imprecision SD (3.13 μm; CV = 0.010), and the inferior quadrant had the thinnest mean TRT (291.55 μm). The quadrant with the lowest (best) imprecision SD was in the nasal one (2.06 μm; CV = 0.007).
conclusionsGood reproducibility was observed for SD-OCT retinal thickness measurements in mice. SD-OCT may be useful for in vivo longitudinal studies in mice.
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