ArticleJournal of clinical microbiology2010
Systematic internal transcribed spacer sequence analysis for identification of clinical mold isolates in diagnostic mycology: a 5-year study.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Oral Candida-biome and Early Childhood Caries: A Systematic Review and Meta-Analysis.International dental journal · 2025Pooled it
- Long-Term Prevalence of Fungal Keratitis at a Swiss Tertiary Eye Clinic.Microorganisms · 2024Article
- Detection of Scedosporium spp.: Colonizer or pathogen? A retrospective analysis of clinical significance and management in a large tertiary center.Medical mycology · 2024Article
- Black grain eumycetoma due toMedical mycology case reports · 2023Article
- Mass spectrometry in research laboratories and clinical diagnostic: a new era in medical mycology.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Review
- Identification of filamentous fungi including dermatophytes using MALDI-TOF mass spectrometry.Folia microbiologica · 2022Article
- Selection of Potential Yeast Probiotics and a Cell Factory for Xylitol or Acid Production from Honeybee Samples.Metabolites · 2021Article
- Molecular detection of fungal pathogens in clinical specimens by 18S rDNA high-throughput screening in comparison to ITS PCR and culture.Scientific reports · 2018Article
- Visible DNA Microarray System as an Adjunctive Molecular Test in Identification of Pathogenic Fungi Directly from a Blood Culture Bottle.Journal of clinical microbiology · 2018Article
- Detection of Bacterial Pathogens from Broncho-Alveolar Lavage by Next-Generation Sequencing.International journal of molecular sciences · 2017Article
- Evaluation of the Bruker Biotyper Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry System for Identification ofFrontiers in microbiology · 2017Article
- Similar efficacy of broad-range ITS PCR and conventional fungal culture for diagnosing fungal infections in non-immunocompromised patients.BMC microbiology · 2016Article
- Genetic diversity of medically important and emerging Candida species causing invasive infection.BMC infectious diseases · 2015Article
- Use of the Bruker MALDI Biotyper for identification of molds in the clinical mycology laboratory.Journal of clinical microbiology · 2014Article
- Molecular identification of clinical "difficult-to-identify" microbes from sequencing 16S ribosomal DNA and internal transcribed spacer 2.Annals of clinical microbiology and antimicrobials · 2014Article
- Article
- Utility of PCR in diagnosis of invasive fungal infections: real-life data from a multicenter study.Journal of clinical microbiology · 2013Article
- Molecular methods for pathogen and microbial community detection and characterization: current and potential application in diagnostic microbiology.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2012Review
- Identification of clinical mold isolates by sequence analysis of the internal transcribed spacer region, ribosomal large-subunit D1/D2, and β-tubulin.Annals of laboratory medicine · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The implementation of internal transcribed spacer (ITS) sequencing for routine identification of molds in the diagnostic mycology laboratory was analyzed in a 5-year study. All mold isolates (n = 6,900) recovered in our laboratory from 2005 to 2009 were included in this study. According to a defined work flow, which in addition to troublesome phenotypic identification takes clinical relevance into account, 233 isolates were subjected to ITS sequence analysis. Sequencing resulted in successful identification for 78.6% of the analyzed isolates (57.1% at species level, 21.5% at genus level). In comparison, extended in-depth phenotypic characterization of the isolates subjected to sequencing achieved taxonomic assignment for 47.6% of these, with a mere 13.3% at species level. Optimization of DNA extraction further improved the efficacy of molecular identification. This study is the first of its kind to testify to the systematic implementation of sequence-based identification procedures in the routine workup of mold isolates in the diagnostic mycology laboratory.
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