ArticleNucleic acids research1991
DNA binding of CPF1 is required for optimal centromere function but not for maintaining methionine prototrophy in yeast.
Article in Nucleic acids research, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 58 citations in OpenAlex.
- An interdependent Cbf1-CCAN interaction stabilizes the budding yeast kinetochore.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- An interdependent Cbf1-CCAN interaction stabilizes the budding yeast kinetochore.bioRxiv : the preprint server for biology · 2026Article
- Keeping it centered: decoding the activities that regulate yeast histone H3 variant Cse4 and confine it to centromeres.Frontiers in cell and developmental biology · 2026Review
- Structural and dynamic mechanisms of CBF3-guided centromeric nucleosome formation.Nature communications · 2021Article
- Article
- Ndc10 is a platform for inner kinetochore assembly in budding yeast.Nature structural & molecular biology · 2011Article
- Endogenous transcription at the centromere facilitates centromere activity in budding yeast.Current biology : CB · 2011Article
- Recognition of the centromere-specific histone Cse4 by the chaperone Scm3.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Molecular structures and interactions in the yeast kinetochore.Cold Spring Harbor symposia on quantitative biology · 2010Article
- SURVEY AND SUMMARY: Saccharomyces cerevisiae basic helix-loop-helix proteins regulate diverse biological processes.Nucleic acids research · 2000Review
- Activator-specific requirement of yeast mediator proteins for RNA polymerase II transcriptional activation.Molecular and cellular biology · 1999Article
- Metabolism of sulfur amino acids in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 1997Review
- Assembly of a bZIP-bHLH transcription activation complex: formation of the yeast Cbf1-Met4-Met28 complex is regulated through Met28 stimulation of Cbf1 DNA binding.The EMBO journal · 1997Article
- Interactions of the yeast centromere and promoter factor, Cpf1p, with the cytochrome c1 upstream region and functional implications on regulated gene expression.Nucleic acids research · 1996Article
- The multifunctional transcription factors Abf1p, Rap1p and Reb1p are required for full transcriptional activation of the chromosomal PGK gene in Saccharomyces cerevisiae.Molecular & general genetics : MGG · 1996Article
- Role of the Saccharomyces cerevisiae general regulatory factor CP1 in methionine biosynthetic gene transcription.Molecular and cellular biology · 1995Article
- Centromere promoter factors (CPF1) of the yeasts Saccharomyces cerevisiae and Kluyveromyces lactis are functionally exchangeable, despite low overall homology.Current genetics · 1994Article
- The vacuolar compartment is required for sulfur amino acid homeostasis in Saccharomyces cerevisiae.Molecular & general genetics : MGG · 1994Article
- SCM2, a tryptophan permease in Saccharomyces cerevisiae, is important for cell growth.Molecular & general genetics : MGG · 1994Article
- Chromatin structure modulation in Saccharomyces cerevisiae by centromere and promoter factor 1.Molecular and cellular biology · 1994Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The centromere and promoter factor 1 (CPF1) binds specifically in vitro and in vivo to an octanucleotide (RTCACRTG). This sequence is found in the centromere DNA element I (CDEI) of yeast centromeres and upstream from a number of transcription units including MET25, GAL2 and TRP1. Inactivation of the CPF1 gene results in three phenotypes; slow growth, a partial loss of centromere function and methionine auxotrophy. These phenotypes correlate well with the known binding sites for CPF1 and have led to the suggestion that CPF1 functions as a kinetochore protein at centromeres and as a transcriptional activator at promoters such as MET25. By analysing transcription from the MET25, GAL2, and TRP1 genes in cpf1 strains, we demonstrate that CPF1 plays no direct role in their transcriptional regulation. Further evidence in support of this comes from the analysis of point mutations in the basic region of CPF1 that affect DNA binding. A strain expressing a non-DNA bound form of CPF1 is phenotypically Met+, shows normal growth rate but has sub-optimal centromere function. We conclude that a DNA-bound form of CPF1 is required for the kinetochore function but not for maintaining methionine prototrophy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.