ArticleMolecular cancer2010
Silencing of TESTIN by dense biallelic promoter methylation is the most common molecular event in childhood acute lymphoblastic leukaemia.
Article in Molecular cancer, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- The value of lung function assessment and Testin expression detection in clinicopathological features and prognosis of NSCLC patients.Journal of cardiothoracic surgery · 2024Article
- The Expression of Testin, Ki-67 and p16 in Cervical Cancer Diagnostics.Current issues in molecular biology · 2023Article
- Silencing of Testin expression is a frequent event in spontaneous lymphomas from Trp53-mutant mice.Scientific reports · 2020Article
- The Role of Testin in Human Cancers.Pathology oncology research : POR · 2019Review
- The PET and LIM1-2 domains of testin contribute to intramolecular and homodimeric interactions.PloS one · 2017Article
- Significance of Inactivated Genes in Leukemia: Pathogenesis and Prognosis.Cell journal · 2017Review
- Testin (Oncology letters · 2016Article
- TESTIN Induces Rapid Death and Suppresses Proliferation in Childhood B Acute Lymphoblastic Leukaemia Cells.PloS one · 2016Article
- Downregulation of TES by hypermethylation in glioblastoma reduces cell apoptosis and predicts poor clinical outcome.European journal of medical research · 2014Article
- Binding of the WASP/N-WASP-interacting protein WIP to actin regulates focal adhesion assembly and adhesion.Molecular and cellular biology · 2014Article
- DNA methylation alterations in grade II- and anaplastic pleomorphic xanthoastrocytoma.BMC cancer · 2014Article
- Widespread resetting of DNA methylation in glioblastoma-initiating cells suppresses malignant cellular behavior in a lineage-dependent manner.Genes & development · 2013Article
- History of Parvovirus B19 infection is associated with a DNA methylation signature in childhood acute lymphoblastic leukemia.Epigenetics · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAberrant promoter DNA methylation has been reported in childhood acute lymphoblastic leukaemia (ALL) and has the potential to contribute to its onset and outcome. However, few reports demonstrate consistent, prevalent and dense promoter methylation, associated with tumour-specific gene silencing. By screening candidate genes, we have detected frequent and dense methylation of the TESTIN (TES) promoter.
resultsBisulfite sequencing showed that 100% of the ALL samples (n = 20) were methylated at the TES promoter, whereas the matched remission (n = 5), normal bone marrow (n = 6) and normal PBL (n = 5) samples were unmethylated. Expression of TES in hyperdiploid, TEL-AML+, BCR-ABL+, and E2A-PBX+ subtypes of B lineage ALL was markedly reduced compared to that in normal bone marrow progenitor cells and in B cells. In addition TES methylation and silencing was demonstrated in nine out of ten independent B ALL propagated as xenografts in NOD/SCID mice.
conclusionIn total, 93% of B ALL samples (93 of 100) demonstrated methylation with silencing or reduced expression of the TES gene. Thus, TES is the most frequently methylated and silenced gene yet reported in ALL. TES, a LIM domain-containing tumour suppressor gene and component of the focal adhesion complex, is involved in adhesion, motility, cell-to-cell interactions and cell signalling. Our data implicate TES methylation in ALL and provide additional evidence for the involvement of LIM domain proteins in leukaemogenesis.
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