ArticleGenome biology2010
Deficiency in mouse Y chromosome long arm gene complement is associated with sperm DNA damage.
Article in Genome biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Large-scale transcriptomic analyses reveal downstream target genes of ZFY1 and ZFY2 transcription factors in male germ cells.Cell death and differentiation · 2026Article
- Return of the forgotten hero: the role of Y chromosome-encoded Zfy in male reproduction.Molecular human reproduction · 2023Article
- DOT1L regulates chromatin reorganization and gene expression during sperm differentiation.EMBO reports · 2023Article
- Two acquired mouse Y chromosome-linked genes, Prssly and Teyorf1, are dispensable for male fertility‡.Biology of reproduction · 2022Article
- Deletion of Orc4 during oogenesis severely reduces polar body extrusion and blocks zygotic DNA replication†.Biology of reproduction · 2022Article
- Review
- Rescue ofGenes · 2019Article
- Horse Y chromosome assembly displays unique evolutionary features and putative stallion fertility genes.Nature communications · 2018Article
- Sequence and Structural Diversity of Mouse Y Chromosomes.Molecular biology and evolution · 2017Article
- SLY regulates genes involved in chromatin remodeling and interacts with TBL1XR1 during sperm differentiation.Cell death and differentiation · 2017Article
- Testicular abnormalities in mice with Y chromosome deficiencies.Biology of reproduction · 2017Article
- Mouse Y-Encoded Transcription Factor Zfy2 Is Essential for Sperm Head Remodelling and Sperm Tail Development.PloS one · 2016Article
- Mouse Y-Encoded Transcription Factor Zfy2 Is Essential for Sperm Formation and Function in Assisted Fertilization.PLoS genetics · 2015Article
- Four core genotypes mouse model: localization of the Sry transgene and bioassay for testicular hormone levels.BMC research notes · 2015Article
- Increasing testicular temperature by exposure to elevated ambient temperatures restores spermatogenesis in adult Utp14b (jsd) mutant (jsd) mice.Andrology · 2015Article
- Increased progesterone production in cumulus-oocyte complexes of female mice sired by males with the Y-chromosome long arm deletion and its potential influence on fertilization efficiency.Reproductive sciences (Thousand Oaks, Calif.) · 2015Article
- SSTY proteins co-localize with the post-meiotic sex chromatin and interact with regulators of its expression.The FEBS journal · 2014Article
- Two Y genes can replace the entire Y chromosome for assisted reproduction in the mouse.Science (New York, N.Y.) · 2014Article
- Deficiency of the multi-copy mouse Y gene Sly causes sperm DNA damage and abnormal chromatin packaging.Journal of cell science · 2013Article
- Human male meiotic sex chromosome inactivation.PloS one · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundMice with severe non-PAR Y chromosome long arm (NPYq) deficiencies are infertile in vivo and in vitro. We have previously shown that sperm from these males, although having grossly malformed heads, were able to fertilize oocytes via intracytoplasmic sperm injection (ICSI) and yield live offspring. However, in continuing ICSI trials we noted a reduced efficiency when cryopreserved sperm were used and with epididymal sperm as compared to testicular sperm. In the present study we tested if NPYq deficiency is associated with sperm DNA damage - a known cause of poor ICSI success.
resultsWe observed that epididymal sperm from mice with severe NPYq deficiency (that is, deletion of nine-tenths or the entire NPYq gene complement) are impaired in oocyte activation ability following ICSI and there is an increased incidence of oocyte arrest and paternal chromosome breaks. Comet assays revealed increased DNA damage in both epididymal and testicular sperm from these mice, with epididymal sperm more severely affected. In all mice the level of DNA damage was increased by freezing. Epididymal sperm from mice with severe NPYq deficiencies also suffered from impaired membrane integrity and abnormal chromatin condensation and suboptimal chromatin protamination. It is therefore likely that the increased DNA damage associated with NPYq deficiency is a consequence of disturbed chromatin remodeling.
conclusionsThis study provides the first evidence of DNA damage in sperm from mice with NPYq deficiencies and indicates that NPYq-encoded gene/s may play a role in processes regulating chromatin remodeling and thus in maintaining DNA integrity in sperm.
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