ArticleTraffic (Copenhagen, Denmark)2010
Spatial regulation of Golgi phosphatidylinositol-4-phosphate is required for enzyme localization and glycosylation fidelity.
Article in Traffic (Copenhagen, Denmark), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
33 citing papers in PubMed, 70 citations in OpenAlex.
- Control of Golgi- V-ATPase through Sac1-dependent co-regulation of PI(4)P and cholesterol.Nature communications · 2025Article
- Orthogonal targeting of SAC1 to mitochondria implicates ORP2 as a major player in PM PI4P turnover.bioRxiv : the preprint server for biology · 2024Article
- Phosphoinositides in New Spaces.Cold Spring Harbor perspectives in biology · 2023Review
- The C10orf76-PI4KB axis orchestrates CERT-mediated ceramide trafficking to the distal Golgi.The Journal of cell biology · 2023Article
- A Plethora of Functions Condensed into Tiny Phospholipids: The Story of PI4P and PI(4,5)PCells · 2023Review
- The intracellular and plasma membrane pools of phosphatidylinositol-4-monophosphate control megakaryocyte maturation and proplatelet formation.Research and practice in thrombosis and haemostasis · 2023Article
- Review
- Imaging of Intracellular and Plasma Membrane Pools of PI(4,5)PLife (Basel, Switzerland) · 2021Article
- GOLPH3 and GOLPH3L are broad-spectrum COPI adaptors for sorting into intra-Golgi transport vesicles.The Journal of cell biology · 2021Article
- The ER cholesterol sensor SCAP promotes CARTS biogenesis at ER-Golgi membrane contact sites.The Journal of cell biology · 2021Article
- Regulation of V-ATPase Activity and Organelle pH by Phosphatidylinositol Phosphate Lipids.Frontiers in cell and developmental biology · 2020Review
- Lipid-dependent coupling of secretory cargo sorting and trafficking at the trans-Golgi network.FEBS letters · 2019Review
- The activity of Sac1 across ER-TGN contact sites requires the four-phosphate-adaptor-protein-1.The Journal of cell biology · 2019Article
- Tied up: Does altering phosphoinositide-mediated membrane trafficking influence neurodegenerative disease phenotypes?Journal of genetics · 2018Review
- SAC-1 ensures epithelial endocytic recycling by restricting ARF-6 activity.The Journal of cell biology · 2018Article
- The phosphoinositide phosphatase Sac1 regulates cell shape and microtubule stability in the developingDevelopment (Cambridge, England) · 2018Article
- SAC1 degrades its lipid substrate PtdIns4eLife · 2018Article
- Structural basis for the binding of tryptophan-based motifs by δ-COP.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Golgi phosphoprotein 3 triggers signal-mediated incorporation of glycosyltransferases into coatomer-coated (COPI) vesicles.The Journal of biological chemistry · 2014Article
- Study of GOLPH3: a potential stress-inducible protein from Golgi apparatus.Molecular neurobiology · 2014Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The enrichment of phosphatidylinositol-4-phosphate (PI(4)P) at the trans Golgi network (TGN) is instrumental for proper protein and lipid sorting, yet how the restricted distribution of PI(4)P is achieved remains unknown. Here, we show that lipid phosphatase Suppressor of actin mutations 1 (SAC1) is crucial for the spatial regulation of Golgi PI(4)P. Ultrastructural analysis revealed that SAC1 is predominantly located at cisternal Golgi membranes but is absent from the TGN, thus confining PI(4)P to the TGN. RNAi-mediated knockdown of SAC1 caused changes in Golgi morphology and mislocalization of Golgi enzymes. Enzymes involved in glycan processing such as mannosidase-II (Man-II) and N-acetylglucosamine transferase-I (GnT-I) redistributed to aberrant intracellular structures and to the cell surface in SAC1 knockdown cells. SAC1 depletion also induced a unique pattern of Golgi-specific defects in N-and O-linked glycosylation. These results indicate that SAC1 organizes PI(4)P distribution between the Golgi complex and the TGN, which is instrumental for resident enzyme partitioning and Golgi morphology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.