ArticleJournal of biophotonics2010
Ultrasensitive label-free photothermal imaging, spectral identification, and quantification of cytochrome c in mitochondria, live cells, and solutions.
Article in Journal of biophotonics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Photothermal Evaluation of Aqueous Magnetite Nanodispersions: Accuracy, Precision, and Limitations.Molecules (Basel, Switzerland) · 2025Article
- Composition of the CytochromeMolecules (Basel, Switzerland) · 2023Article
- Towards rainbow portable Cytophone with laser diodes for global disease diagnostics.Scientific reports · 2022Article
- Label-free dynamic imaging of mitochondria and lysosomes within living cells via simultaneous dual-pump photothermal microscopy.Biomedical optics express · 2019Article
- Photothermal imaging of skeletal muscle mitochondria.Biomedical optics express · 2017Article
- Label-free photoacoustic tomography of whole mouse brain structuresNeurophotonics · 2016Article
- Fast 3D visualization of endogenous brain signals with high-sensitivity laser scanning photothermal microscopy.Biomedical optics express · 2016Article
- Reduction of distortion in photothermal microscopy and its application to the high-resolution three-dimensional imaging of nonfluorescent tissues.Biomedical optics express · 2015Article
- Photothermal confocal multicolor microscopy of nanoparticles and nanodrugs in live cells.Drug metabolism reviews · 2015Review
- Label-free photoacoustic nanoscopy.Journal of biomedical optics · 2014Article
- Multi-modal label-free imaging based on a femtosecond fiber laser.Biomedical optics express · 2014Article
- Photoacoustic and photothermal detection of circulating tumor cells, bacteria and nanoparticles in cerebrospinal fluid in vivo and ex vivo.Journal of biophotonics · 2013Article
- Label-free photoacoustic microscopy of cytochromes.Journal of biomedical optics · 2013Article
- Photothermal confocal spectromicroscopy of multiple cellular chromophores and fluorophores.Biophysical journal · 2012Article
- In vivo photoacoustic and photothermal cytometry for monitoring multiple blood rheology parameters.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2011Review
- Photothermal multispectral image cytometry for quantitative histology of nanoparticles and micrometastasis in intact, stained and selectively burned tissues.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2010Article
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Authors and funding
7 authors.
Funding
Abstract
Light-absorbing endogenous cellular proteins, in particular cytochrome c, are used as intrinsic biomarkers for studies of cell biology and environment impacts. To sense cytochrome c against real biological backgrounds, we combined photothermal (PT) thermal-lens single-channel schematic in a back-synchronized measurement mode and a multiplex thermal-lens schematic in a transient high resolution (ca. 350 nm) imaging mode. These multifunctional PT techniques using continuous-wave (cw) Ar+ laser and a nanosecond pulsed optical parametric oscillator in the visible range demonstrated the capability for label-free spectral identification and quantification of trace amounts of cytochrome c in a single mitochondrion alone or within a single live cell. PT imaging data were verified in parallel by molecular targeting and fluorescent imaging of cellular cytochrome c. The detection limit of cytochrome c in a cw mode was 5 x 10(-9) mol/L (80 attomols in the signal-generation zone); that is ca. 10³ lower than conventional absorption spectroscopy. Pulsed fast PT microscopy provided the detection limit for cytochrome c at the level of 13 zmol (13 x 10(-21) mol) in the ultrasmall irradiated volumes limited by optical diffraction effects. For the first time, we demonstrate a combination of high resolution PT imaging with PT spectral identification and ultrasensitive quantitative PT characterization of cytochrome c within individual mitochondria in single live cells. A potential of far-field PT microscopy to sub-zeptomol detection thresholds, resolution beyond diffraction limit, PT Raman spectroscopy, and 3D imaging are further highlighted.
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