ArticleNucleic acids research2010
Time-dependent increase in ribosome processivity.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- NNature · 2026Article
- Article
- Deubiquitinase OTUD1 Resolves Stalled Translation on polyA and Rare Codon Rich mRNAs.Molecular and cellular biology · 2022Article
- Luciferase-based reporter system for in vitro evaluation of elongation rate and processivity of ribosomes.Nucleic acids research · 2021Article
- Single-molecule imaging analysis reveals the mechanism of a high-catalytic-activity mutant of chitinase A fromThe Journal of biological chemistry · 2020Article
- mTORC1 Enhances Early Phase Ribosome Processivity.Frontiers in molecular biosciences · 2020Article
- Intrinsically Disordered Linkers Impart Processivity on Enzymes by Spatial Confinement of Binding Domains.International journal of molecular sciences · 2019Article
- The Rio1p ATPase hinders premature entry into translation of late pre-40S pre-ribosomal particles.Nucleic acids research · 2017Article
- In vitro labeling strategies for in cellulo fluorescence microscopy of single ribonucleoprotein machines.Protein science : a publication of the Protein Society · 2017Article
- N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density.Nucleic acids research · 2017Article
- Polysomes of Trypanosoma brucei: Association with Initiation Factors and RNA-Binding Proteins.PloS one · 2015Article
- Probing the closed-loop model of mRNA translation in living cells.RNA biology · 2015Article
- Detecting translational regulation by change point analysis of ribosome profiling data sets.RNA (New York, N.Y.) · 2014Article
- In vitro human cell-free expression system for synthesis of malaria proteins.Parasitology research · 2012Article
- The DEAD-box protein Dhh1 promotes decapping by slowing ribosome movement.PLoS biology · 2012Article
- Animal virus schemes for translation dominance.Current opinion in virology · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We created a novel tripartite reporter RNA to separately and simultaneously examine ribosome translation rates at the 5'- and 3'-ends of a large open reading frame (ORF) in vitro in HeLa cell lysates. The construct contained Renilla luciferase (RLuc), β-galactosidase and firefly luciferase (FLuc) ORFs linked in frame and separated by a viral peptide sequence that causes cotranslational scission of emerging peptide chains. The length of the ORF contributed to low ribosome processivity, a low number of initiating ribosomes completing translation of the entire ORF. We observed a time-dependent increase in FLuc production rate that was dependent on a poly(A) tail and poly(A)-binding protein, but was independent of eIF4F function. Stimulation of FLuc production occurred earlier on shorter RNA templates. Cleavage of eIF4G at times after ribosome loading on templates occurred did not cause immediate cessation of 5'-RLuc translation; rather, a delay was observed that shortened when shorter templates were translated. Electron microscopic analysis of polysome structures in translation lysates revealed a time-dependent increase in ribosome packing and contact that correlated with increased processivity on the FLuc ORF. The results suggest that ORF transit combined with PABP function contribute to interactions between ribosomes that increase or sustain processivity on long ORFs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.