ArticleMolecular human reproduction2010
Association between amino acid turnover and chromosome aneuploidy during human preimplantation embryo development in vitro.
Article in Molecular human reproduction, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 120 citations in OpenAlex.
- Endometrial stromal cells of women with recurrent miscarriage fail to discriminate between high- and low-quality human embryos.PloS one · 2012Trial
- "Old and new" frontiers in embryo viability assessment: current practices and introduction of emerging approaches.Biology of reproduction · 2026Review
- Heat Shock Affects Amino Acid Metabolism in Bovine Cumulus Cells and Denuded Oocytes During In Vitro Maturation.Biology · 2026Article
- Exploring the increasing aneuploidy stress effects on embryo morphokinetic development: non-linear and stage-specific disruption in developmental timing.Journal of assisted reproduction and genetics · 2026Article
- When less really is more: reducing embryo manipulation to lower metabolic stress and improving long term outcomes.Reproduction, fertility, and development · 2026Review
- Delayed blastocyst development is associated with altered metabolism and proteome in male and female bovine embryos†.Biology of reproduction · 2025Article
- Inferring metabolic objectives and trade-offs in single cells during embryogenesis.Cell systems · 2025Article
- Abnormal amino acid synthesis and glutathione metabolism may affect PCOS blastocyst development: an examination of in vitro mouse blastocysts model utilizing RNA-sequencing.BMC endocrine disorders · 2024Article
- Impact of preovulatory follicle maturity on oocyte metabolism and embryo development.PNAS nexus · 2024Article
- Bovine embryo productionAnimal reproduction · 2024Review
- Article
- Metabolic Signature of Energy Metabolism Alterations and Excess Nitric Oxide Production in Culture Media Correlate with Low Human Embryo Quality and Unsuccessful Pregnancy.International journal of molecular sciences · 2023Article
- Glutamine as a Potential Noninvasive Biomarker for Human Embryo Selection.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
- Epigenetic Risks of Medically Assisted Reproduction.Journal of clinical medicine · 2022Review
- Microphysiological stem cell models of the human heart.Materials today. Bio · 2022Review
- LC-MS Analysis Revealed the Significantly Different Metabolic Profiles in Spent Culture Media of Human Embryos with Distinct Morphology, Karyotype and Implantation Outcomes.International journal of molecular sciences · 2022Article
- The Quiet Embryo Hypothesis: 20 years on.Frontiers in physiology · 2022Review
- Mitochondrial content, activity, and morphology in prepubertal and adult human ovaries.Journal of assisted reproduction and genetics · 2021Article
- Amino Acids and the Early Mammalian Embryo: Origin, Fate, Function and Life-Long Legacy.International journal of environmental research and public health · 2021Review
- The Metabolic Signature of In Vitro Produced Bovine Embryos Helps Predict Pregnancy and Birth after Embryo Transfer.Metabolites · 2021Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
Abstract
This study investigated the relationship between human preimplantation embryo metabolism and aneuploidy rates during development in vitro. One hundred and eighty-eight fresh and cryopreserved embryos from 59 patients (33.9 +/- 0.6 years) were cultured for 2-5 days. The turnover of 18 amino acids was measured in spent media by high-performance liquid chromatography. Embryos were either fixed for interphase fluorescent in situ hybridization analysis of chromosomes 13, 18, 19, 21, X or Y, or were assayed for mitochondrial activity. Amino acid turnover was different (P < 0.05) between stage-matched fresh and cryopreserved embryos due to blastomere loss following warming. The proportion of embryos with aneuploid cells increased as cell division progressed from pronucleate- (23%) to late cleavage stages (50-70%). Asparagine, glycine and valine turnover was significantly different between uniformly genetically normal and uniformly abnormal embryos on Days 2-3 of culture. By Days 3-4, the profiles of serine, leucine and lysine differed between uniformly euploid versus aneuploid embryos. Gender significantly (P < 0.05) affected the metabolism of tryptophan, leucine and asparagine by cleavage-stage embryos. Pronucleate zygotes had a significantly higher proportion of active:inactive mitochondria compared with cleavage-stage embryos. Furthermore, mitochondrial activity was correlated (P < 0.05) with altered aspartate and glutamine turnover. These results demonstrate the association between the metabolism, cytogenetic composition and health of human embryos in vitro.
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Registered trials
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