ArticleMolecular & cellular proteomics : MCP2010
Sialic acid-focused quantitative mouse serum glycoproteomics by multiple reaction monitoring assay.
Article in Molecular & cellular proteomics : MCP, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 86 citations in OpenAlex.
- Development and validation of a method for analyzing the sialylated glycopeptides of recombinant erythropoietin in urine using LC-HRMS.Scientific reports · 2023Article
- Methods for quantification of glycopeptides by liquid separation and mass spectrometry.Mass spectrometry reviews · 2023Review
- Mouse tissue glycome atlas 2022 highlights inter-organ variation in major N-glycan profiles.Scientific reports · 2022Article
- Selective reaction monitoring approach using structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics.RSC advances · 2022Article
- Selective Enrichment of Sialylglycopeptides Enabled by Click Chemistry and Dynamic Covalent Exchange.Analytical chemistry · 2022Article
- PRM-MS Quantitative Analysis of IsomericMetabolites · 2021Article
- Generating orthogonal glycosyltransferase and nucleotide sugar pairs as next-generation glycobiology tools.Current opinion in chemical biology · 2021Review
- Review
- Recent advances in mass spectrometry (MS)-based glycoproteomics in complex biological samples.Trends in analytical chemistry : TRAC · 2019Article
- Global and site-specific analysis of protein glycosylation in complex biological systems with Mass Spectrometry.Mass spectrometry reviews · 2019Review
- Isolation and characterization of glycosylated neuropeptides.Methods in enzymology · 2019Article
- Synthetic Glycopeptides Allow for the Quantitation of Scarce Nonfucosylated IgG FcACS medicinal chemistry letters · 2018Article
- Mass Spectrometry Approaches to Glycomic and Glycoproteomic Analyses.Chemical reviews · 2018Review
- Synthetic glycopeptides as a designated standard in focused glycoproteomics to discover serum cancer biomarkers.MedChemComm · 2018Article
- Changes in the expression of N- and O-glycopeptides in patients with colorectal cancer and hepatocellular carcinoma quantified by full-MS scan FT-ICR and multiple reaction monitoring.Journal of proteomics · 2017Article
- Analysis of Permethylated Glycan by Liquid Chromatography (LC) and Mass Spectrometry (MS).Methods in molecular biology (Clifton, N.J.) · 2017Article
- Chemical Glycoproteomics.Chemical reviews · 2016Review
- Terminal Galactosylation and Sialylation Switching on Membrane Glycoproteins upon TNF-Alpha-Induced Insulin Resistance in Adipocytes.Molecular & cellular proteomics : MCP · 2016Article
- A Method for Comprehensive Glycosite-Mapping and Direct Quantitation of Serum Glycoproteins.Journal of proteome research · 2015Article
- Quantitation of permethylated N-glycans through multiple-reaction monitoring (MRM) LC-MS/MS.Journal of the American Society for Mass Spectrometry · 2015Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite increasing importance of protein glycosylation, most of the large-scale glycoproteomics have been limited to profiling the sites of N-glycosylation. However, in-depth knowledge of protein glycosylation to uncover functions and their clinical applications requires quantitative glycoproteomics eliciting both peptide and glycan sequences concurrently. Here we describe a novel strategy for the multiplexed quantitative mouse serum glycoproteomics based on a specific chemical ligation, namely, reverse glycoblotting technique, focusing sialic acids and multiple reaction monitoring (MRM). LC-MS/MS analysis of de-glycosylated peptides identified 270 mouse serum peptides (95 glycoproteins) as sialylated glycopeptides, of which 67 glycopeptides were fully characterized by MS/MS analyses in a straightforward manner. We revealed the importance of a fragment ion containing innermost N-acetylglucosamine (GlcNAc) residue as MRM transitions regardless the sequence of the peptides. Versatility of the reverse glycoblotting-assisted MRM assays was demonstrated by quantitative comparison of 25 targeted glycopeptides from 16 proteins between mice with homo and hetero types of diabetes disease model.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.