ArticleMolecular and biochemical parasitology2010
Transcriptome analyses of the Giardia lamblia life cycle.
Article in Molecular and biochemical parasitology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 68 citations in OpenAlex.
- Pathogenicity and virulence ofVirulence · 2026Review
- Giardia fibrillarin: a bioinformatics exploration of sequence and structure.Journal of applied genetics · 2025Article
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- Characterization of Metronidazole-ResistantFrontiers in microbiology · 2022Article
- A Detailed Gene Expression Map ofGenes · 2021Article
- Click chemistry-facilitated comprehensive identification of proteins adducted by antimicrobial 5-nitroimidazoles for discovery of alternative drug targets against giardiasis.PLoS neglected tropical diseases · 2020Article
- Role of gamma-giardin in ventral disc formation of Giardia lamblia.Parasites & vectors · 2019Article
- Sense-antisense gene overlap is probably a cause for retaining the few introns in Giardia genome and the implications.Biology direct · 2018Article
- Glucosylceramide transferase in Giardia preferentially catalyzes the synthesis of galactosylceramide during encystation.Molecular and biochemical parasitology · 2017Article
- Article
- Constitutive aneuploidy and genomic instability in the single-celled eukaryote Giardia intestinalis.MicrobiologyOpen · 2016Article
- Coordinated Changes in Gene Expression Throughout Encystation of Giardia intestinalis.PLoS neglected tropical diseases · 2016Article
- Sphingolipids, Lipid Rafts, and Giardial Encystation: The Show Must Go On.Current tropical medicine reports · 2015Article
- Identification of Giardia lamblia DHHC proteins and the role of protein S-palmitoylation in the encystation process.PLoS neglected tropical diseases · 2014Article
- Article
- The proteome landscape of Giardia lamblia encystation.PloS one · 2013Article
- Transcriptome profiling of Giardia intestinalis using strand-specific RNA-seq.PLoS computational biology · 2013Article
- Putative SF2 helicases of the early-branching eukaryote Giardia lamblia are involved in antigenic variation and parasite differentiation into cysts.BMC microbiology · 2012Article
- Importance of enolase in Giardia lamblia differentiation.Molecular and biochemical parasitology · 2012Article
- Plasmid vectors for proteomic analyses in Giardia: purification of virulence factors and analysis of the proteasome.Eukaryotic cell · 2012Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
We quantified mRNA abundance from 10 stages in the Giardia lamblia life cycle in vitro using Serial Analysis of Gene Expression (SAGE). 163 abundant transcripts were expressed constitutively. 71 transcripts were upregulated specifically during excystation and 42 during encystation. Nonetheless, the transcriptomes of cysts and trophozoites showed major differences. SAGE detected co-expressed clusters of 284 transcripts differentially expressed in cysts and excyzoites and 287 transcripts in vegetative trophozoites and encysting cells. All clusters included known genes and pathways as well as proteins unique to Giardia or diplomonads. SAGE analysis of the Giardia life cycle identified a number of kinases, phosphatases, and DNA replication proteins involved in excystation and encystation, which could be important for examining the roles of cell signaling in giardial differentiation. Overall, these data pave the way for directed gene discovery and a better understanding of the biology of G. lamblia.
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