ArticleBMC biology2010
Characterization of a heat resistant beta-glucosidase as a new reporter in cells and mice.
Article in BMC biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- PtBGL: a cost-effective alternative to GUS reporter with applications in plant imprint dyeing.Frontiers in plant science · 2025Article
- Retinoic Acid Is Required for Neural Stem and Progenitor Cell Proliferation in the Adult Hippocampus.Stem cell reports · 2018Article
- Wnt ligands influence tumour initiation by controlling the number of intestinal stem cells.Nature communications · 2018Article
- Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods.Breast cancer research : BCR · 2016Article
- Single-cell lineage tracing in the mammary gland reveals stochastic clonal dispersion of stem/progenitor cell progeny.Nature communications · 2016Article
- Retinoic acid regulates embryonic development of mammalian submandibular salivary glands.Developmental biology · 2015Article
- A reporter mouse line with doxycyclin-inducible expression of β-glucosidase.Histochemistry and cell biology · 2014Article
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Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 5 institutions in 1 country.
Funding
Abstract
backgroundReporter genes are widely used in biology and only a limited number are available. We present a new reporter gene for the localization of mammalian cells and transgenic tissues based on detection of the bglA (SYNbglA) gene of Caldocellum saccharolyticum that encodes a thermophilic beta-glucosidase.
resultsSYNbglA was generated by introducing codon substitutions to remove CpG motifs as these are associated with gene silencing in mammalian cells. SYNbglA expression can be localized in situ or detected quantitatively in colorimetric assays and can be co-localized with E. coli beta-galactosidase. Further, we have generated a Cre-reporter mouse in which SYNbglA is expressed following recombination to demonstrate the general utility of SYNbglA for in vivo analyses. SYNbglA can be detected in tissue wholemounts and in frozen and wax embedded sections.
conclusionsSYNbglA will have general applicability to developmental and molecular studies in vitro and in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.