Evidence map›Paper›PMID 20569215›Full record

ArticleBioTechniques2010

Dual primer emulsion PCR for next- generation DNA sequencing.

Ming Yan Xu, Anthony D Aragon, Monica R Mascarenas, Norah Torrez-Martinez, Jeremy S Edwards

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioTechniques, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ming Yan XuMolecular Genetics and Microbiology, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
Anthony D Aragon
Monica R Mascarenas
Norah Torrez-Martinez
Jeremy S Edwards

Funding

Polony Sequencing and the $1000 Genome R01HG005852 · NHGRI · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI EDWARDS, JEREMY S · 2010 to 2012
$2.7M
Polony Sequencing the Human GenomeR21HG004350 · NHGRI · UNIVERSITY OF NEW MEXICO · PI EDWARDS, JEREMY S · 2007 to 2009
$900k
Sequencing Chr. 6 in Melanoma PatientsR21CA125397 · NCI · UNIVERSITY OF NEW MEXICO · PI EDWARDS, JEREMY S · 2008 to 2009
$371k
NCI NIH HHS R21 CA125397NHGRI NIH HHS R01 HG005852NHGRI NIH HHS R21 HG004350NHGRI NIH HHS R21HG004350
6 · The paper itself

Abstract

We have developed a highly sensitive single-molecule clonal amplification method called dual primer emulsion PCR (DPePCR) for next-generation DNA sequencing. The approach is similar in concept to standard emulsion PCR; however, in DPePCR both primers are attached to the beads, therefore following PCR amplification, both strands of the PCR products are attached to the beads. The ends of each strand can be freed for analysis by restriction digestion of the bridged PCR fragments, which allows efficient paired-end sequencing of fragment libraries.

Indexed as

DNA PrimersEmulsionsPolymerase Chain ReactionReproducibility of ResultsSequence Analysis, DNAStreptococcus pyogenesDNA PrimersEmulsions

Identifiers

PMID20569215
PMCPMC4049327

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.