ArticleMolecular pharmaceutics2010
Effect of cholesterol nanodomains on the targeting of lipid-based gene delivery in cultured cells.
Article in Molecular pharmaceutics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- A lipid nanoparticle platform incorporating trehalose glycolipid for exceptional mRNA vaccine safety.Bioactive materials · 2024Article
- Physicochemical Factors That Influence the Biocompatibility of Cationic Liposomes and Their Ability to Deliver DNA to the Nuclei of Ovarian Cancer SK-OV-3 Cells.Materials (Basel, Switzerland) · 2021Article
- Anti-F1000Research · 2020Article
- A novel method for conjugating the terminal amine of peptide ligands to cholesterol: synthesis iRGD-cholesterol.Therapeutic delivery · 2019Article
- Nanoparticle uptake by circulating leukocytes: A major barrier to tumor delivery.Journal of controlled release : official journal of the Controlled Release Society · 2018Article
- Interfacially Engineered Pyridinium Pseudogemini Surfactants as Versatile and Efficient Supramolecular Delivery Systems for DNA, siRNA, and mRNA.ACS applied materials & interfaces · 2017Article
- Intracellular trafficking of hybrid gene delivery vectors.Journal of controlled release : official journal of the Controlled Release Society · 2015Article
- Relating toxicity to transfection: using sphingosine to maintain prolonged expression in vitro.Molecular pharmaceutics · 2015Article
- Stability and toxicity of empty or gene-loaded lipopolysaccharide-amine nanopolymersomes.International journal of nanomedicine · 2015Article
- Examination of the specificity of tumor cell derived exosomes with tumor cells in vitro.Biochimica et biophysica acta · 2014Article
- Mechanistic evaluation of the transfection barriers involved in lipid-mediated gene delivery: interplay between nanostructure and composition.Biochimica et biophysica acta · 2014Article
- Self-assembled micelles of novel amphiphilic copolymer cholesterol-coupled F68 containing cabazitaxel as a drug delivery system.International journal of nanomedicine · 2014Article
- Questioning the Use of PEGylation for Drug Delivery.Drug delivery and translational research · 2013Article
- The effects of lipoplex formulation variables on the protein corona and comparisons with in vitro transfection efficiency.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- Design of hybrid lipid/retroviral-like particle gene delivery vectors.Molecular pharmaceutics · 2013Article
- Cholesterol domains enhance transfection.Therapeutic delivery · 2013Article
- Quantitative silencing of EGFP reporter gene by self-assembled siRNA lipoplexes of LinOS and cholesterol.Molecular pharmaceutics · 2012Article
- Ligands located within a cholesterol domain enhance gene delivery to the target tissue.Journal of controlled release : official journal of the Controlled Release Society · 2012Article
- The effect of cholesterol domains on PEGylated liposomal gene delivery in vitro.Therapeutic delivery · 2011Article
- Drug delivery trends in clinical trials and translational medicine: challenges and opportunities in the delivery of nucleic acid-based therapeutics.Journal of pharmaceutical sciences · 2011Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Targeted gene delivery offers immense potential for clinical applications. Liposomes decorated with targeting ligands have been extensively used for both in vitro and in vivo gene delivery. Lipoplexes with high cholesterol content that result in cholesterol domain formation within the complexes have been shown to exhibit enhanced transfection in vitro and resistance to serum-induced aggregation. In the present study, folate was employed as a targeting ligand that was conjugated with either cholesterol or a diacyl lipid (DSPE), and these conjugates were incorporated into lipoplexes formulated with DOTAP/cholesterol (wt/wt: 31/69) that are known to possess cholesterol nanodomains. Cellular uptake and transfection of these lipoplexes in the presence of 50% serum were examined when the ligand was located within or excluded from the cholesterol nanodomain. Lipoplexes with folate-cholesterol exhibited a 50-fold increase in transfection compared to those with folate-DSPE, while the cellular uptake level is only 40% of that with folate-DSPE. These results indicate that the presence of the ligand within the cholesterol domain promotes more productive transfection in cultured cells, and intracellular trafficking of the lipoplexes after entry into cells plays a crucial role in gene delivery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.