ArticleTransgenic research2011
A barley cysteine-proteinase inhibitor reduces the performance of two aphid species in artificial diets and transgenic Arabidopsis plants.
Article in Transgenic research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 99 citations in OpenAlex.
- Revealing Genomic Traits and Evolutionary Insights of Oryza officinalis from Southern China Through Genome Assembly and Transcriptome Analysis.Rice (New York, N.Y.) · 2025Article
- Molecular Interactions Between Plants and Aphids: Recent Advances and Future Perspectives.Insects · 2024Review
- Identification of Markers Associated with Wheat Dwarf Virus (WDV) Tolerance/Resistance in Barley (Viruses · 2023Article
- Silencing a Myzus persicae Macrophage Inhibitory Factor by Plant-Mediated RNAi Induces Enhanced Aphid Mortality Coupled with Boosted RNAi Efficacy in Transgenic Potato Lines.Molecular biotechnology · 2022Article
- Proteinase inhibitors in legume herbivore defense: from natural to genetically engineered protectants.Plant cell reports · 2022Review
- Genome-Wide Identification and Characterization of theInternational journal of molecular sciences · 2021Article
- Purification and Characterization of a Novel Thermostable Papain Inhibitor fromPharmaceutics · 2021Article
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- Differential response of silencing HvIcy2 barley plants against Magnaporthe oryzae infection and light deprivation.BMC plant biology · 2018Article
- Overexpression of HvIcy6 in Barley Enhances Resistance against Tetranychus urticae and Entails Partial Transcriptomic Reprogramming.International journal of molecular sciences · 2018Article
- Can Plant Defence Mechanisms Provide New Approaches for the Sustainable Control of the Two-Spotted Spider Mite Tetranychus urticae?International journal of molecular sciences · 2018Review
- In Vitro and In Vivo Effects of α-Amylase Inhibitor From Avena sativa Seeds on Life History and Physiological Characteristics of Sitotroga cerealella (Lepidoptera: Gelechiidae).Journal of insect science (Online) · 2017Article
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- The Protease Inhibitor CI2c Gene Induced by Bird Cherry-Oat Aphid in Barley Inhibits Green Peach Aphid Fecundity in Transgenic Arabidopsis.International journal of molecular sciences · 2017Article
- Transgenic sugarcane overexpressing CaneCPI-1 negatively affects the growth and development of the sugarcane weevil Sphenophorus levis.Plant cell reports · 2017Article
- Functional proteomics-aided selection of protease inhibitors for herbivore insect control.Scientific reports · 2016Article
- Phytocystatins: Defense Proteins against Phytophagous Insects and Acari.International journal of molecular sciences · 2016Review
- Modification of Cry4Aa toward Improved Toxin Processing in the Gut of the Pea Aphid, Acyrthosiphon pisum.PloS one · 2016Article
- Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cystatins from plants have been implicated in plant defense towards insects, based on their role as inhibitors of heterologous cysteine-proteinases. We have previously characterized thirteen genes encoding cystatins (HvCPI-1 to HvCPI-13) from barley (Hordeum vulgare), but only HvCPI-1 C68 → G, a variant generated by direct-mutagenesis, has been tested against insects. The aim of this study was to analyze the effects of the whole gene family members of barley cystatins against two aphids, Myzus persicae and Acyrthosiphon pisum. All the cystatins, except HvCPI-7, HvCPI-10 and HvCPI-12, inhibited in vitro the activity of cathepsin L- and/or B-like proteinases, with HvCPI-6 being the most effective inhibitor for both aphid species. When administered in artificial diets, HvCPI-6 was toxic to A. pisum nymphs (LC(50) = 150 μg/ml), whereas no significant mortality was observed on M. persicae nymphs up to 1000 μg/ml. The effects of HvCPI-6 ingestion on A. pisum were correlated with a decrease of cathepsin B- and L-like proteinase activities. In the case of M. persicae, there was an increase of these proteolytic activities, but also of the aminopeptidase-like activity, suggesting that this species is regulating both target and insensitive enzymes to overcome the effects of the cystatin. To further analyze the potential of barley cystatins as insecticidal proteins against aphids, Arabidopsis plants expressing HvCPI-6 were tested against M. persicae. For A. pisum, which does not feed on Arabidopsis, a combined diet-Vicia faba plant bioassay was performed. A significant delay in the development time to reach the adult stage was observed in both species. The present study demonstrates the potential of barley cystatins to interfere with the performance of two aphid species.
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