ArticleInternational journal of legal medicine2011
First experiences using the new Powerplex® ESX17 and ESI17 kits in casework analysis and allele frequencies for two different regions in Germany.
Article in International journal of legal medicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 27 citations in OpenAlex.
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- Impact of several wearers on the persistence of DNA on clothes-a study with experimental scenarios.International journal of legal medicine · 2018Article
- Persistence of DNA on clothes after exposure to water for different time periods-a study on bathtub, pond, and river.International journal of legal medicine · 2018Article
- Does zero really mean nothing?-first experiences with the new PowerQuant(TM) system in comparison to established real-time quantification kits.International journal of legal medicine · 2016Article
- Population data for 22 autosomal STR loci from Estonia.International journal of legal medicine · 2015Article
- Population data for 15 autosomal STR loci from Latvia.International journal of legal medicine · 2015Article
- Everything clean? Transfer of DNA traces between textiles in the washtub.International journal of legal medicine · 2015Article
- Influence of an individual's age on the amount and interpretability of DNA left on touched items.International journal of legal medicine · 2013Article
- The new guidelines for paternity analysis in Germany-how many STR loci are necessary when investigating duo cases?International journal of legal medicine · 2013Article
- Allele frequencies for the 16 short tandem repeats of the Powerplex ESX17 kit in a population from Turkey.International journal of legal medicine · 2013Article
- The SNPforID Assay as a Supplementary Method in Kinship and Trace Analysis.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2012Article
- Highly efficient nuclear DNA typing of the World War II skeletal remains using three new autosomal short tandem repeat amplification kits with the extended European Standard Set of loci.Croatian medical journal · 2012Article
- Good shedder or bad shedder--the influence of skin diseases on forensic DNA analysis from epithelial abrasions.International journal of legal medicine · 2012Article
- The new Powerplex® ESX17 and ESI17 kits in paternity and maternity analyses involving people from Africa--including allele frequencies for three African populations.International journal of legal medicine · 2011Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA databases are the most efficient tools in criminal investigations with unknown perpetrators. Due to a significant number of random matches in cross-border DNA profile exchanges, the European Network of Forensic Science Institutes (ENFSI) proposed the addition of further short tandem repeats (STRs) to European DNA databases. Therefore, the new Powerplex® ESX17 and Powerplex® ESI17 kits from Promega comprised the 11 established DNA database STRs and additionally the well-known loci D1S1656 and D12S391, as well as D2S441, D10S1248, and D22S1045. The latter three STRs are thereby established as so-called mini-STRs to fulfill the increasing requirements regarding sensitivity and reproducibility for analysis of minute amounts of DNA. Here, we provide allele frequencies for the five additional STRs from two populations from Germany. A test regarding suitability and robustness of the new kits for routine trace analysis showed that it is more likely to obtain a meaningful profile using Powerplex® ESX17 and Powerplex® ESI17 kits compared to the Powerplex® ES kit. However, for both new kits the range of template DNA amount is rather small, e.g., slightly more DNA than recommended resulted in DNA profiles which could not be reliably evaluated due to allelic drop-in or imbalances and overshoots. In our opinion, the new kits are very promising new tools in forensic trace analysis even though handling and evaluation should yet be carried out with great caution.
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