ArticleMolecular and cellular biology2010
In vitro reconstitution of PHO5 promoter chromatin remodeling points to a role for activator-nucleosome competition in vivo.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Nucleosome Remodeling at the YeastInternational journal of molecular sciences · 2023Article
- Article
- Role of transcription factor-mediated nucleosome disassembly in PHO5 gene expression.Scientific reports · 2016Article
- Single-cell nucleosome mapping reveals the molecular basis of gene expression heterogeneity.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The RSC chromatin remodeling complex has a crucial role in the complete remodeler set for yeast PHO5 promoter opening.Nucleic acids research · 2014Article
- The yeast PHO5 promoter: from single locus to systems biology of a paradigm for gene regulation through chromatin.Nucleic acids research · 2014Review
- Article
- DNA sequence preferences of transcriptional activators correlate more strongly than repressors with nucleosomes.Molecular cell · 2012Article
- Dynamic changes in nucleosome occupancy are not predictive of gene expression dynamics but are linked to transcription and chromatin regulators.Molecular and cellular biology · 2012Article
- Targeted INO80 enhances subnuclear chromatin movement and ectopic homologous recombination.Genes & development · 2012Article
- Histone H3 lysine 4 hypermethylation prevents aberrant nucleosome remodeling at the PHO5 promoter.Molecular and cellular biology · 2011Article
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Authors and funding
6 authors.
Funding
Abstract
The yeast PHO5 promoter is a classical model for studying the role of chromatin in gene regulation. To enable biochemical dissection of the mechanism leading to PHO5 activation, we reconstituted the process in vitro. Positioned nucleosomes corresponding to the repressed PHO5 promoter state were assembled using a yeast extract-based in vitro system. Addition of the transactivator Pho4 yielded an extensive DNase I-hypersensitive site resembling induced PHO5 promoter chromatin. Importantly, this remodeling was energy dependent. In contrast, little or no chromatin remodeling was detected at the PHO8 or PHO84 promoter in this in vitro system. Only the PHO5 promoter harbors a high-affinity intranucleosomal Pho4 binding site (UASp) where Pho4 binding can compete with nucleosome formation, prompting us to test the importance of such competition for chromatin remodeling by analysis of UASp mutants in vivo. Indeed, the intranucleosomal location of the UASp element was critical, but not essential, for complete remodeling at the PHO5 promoter in vivo. Further, binding of just the Gal4 DNA binding domain to an intranucleosomal site could increase PHO5 promoter opening. These data establish an auxiliary role for DNA binding competition between Pho4 and histones in PHO5 promoter chromatin remodeling in vivo.
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