ArticleInfection and immunity2010
Global gene expression profiling of Yersinia pestis replicating inside macrophages reveals the roles of a putative stress-induced operon in regulating type III secretion and intracellular cell division.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Characterization of YhcN in stress adaptation and its complex transcriptional regulation by SlyA inFrontiers in cellular and infection microbiology · 2026Article
- Transcriptome analysis ofmBio · 2024Article
- Article
- Small Insertions and Deletions Drive Genomic Plasticity during Adaptive Evolution of Yersinia pestis.Microbiology spectrum · 2022Review
- Secretory System Components as Potential Prophylactic Targets for Bacterial Pathogens.Biomolecules · 2021Review
- The Diverse Roles of the Global Transcriptional Regulator PhoP in the Lifecycle ofPathogens (Basel, Switzerland) · 2020Review
- Putative Horizontally Acquired Genes, Highly Transcribed during Yersinia pestis Flea Infection, Are Induced by Hyperosmotic Stress and Function in Aromatic Amino Acid Metabolism.Journal of bacteriology · 2020Article
- Co-infection Assay to Determine Yersinia pestis Competitive Fitness in Fleas.Methods in molecular biology (Clifton, N.J.) · 2019Article
- A Single Amino Acid Change in the Response Regulator PhoP, Acquired during Yersinia pestis Evolution, Affects PhoP Target Gene Transcription and Polymyxin B Susceptibility.Journal of bacteriology · 2018Article
- Cell-free Determination of Binary Complexes That Comprise Extended Protein-Protein Interaction Networks of Yersinia pestis.Molecular & cellular proteomics : MCP · 2016Article
- Environmental Regulation of Yersinia Pathophysiology.Frontiers in cellular and infection microbiology · 2016Review
- Transcriptomic Analysis of Yersinia enterocolitica Biovar 1B Infecting Murine Macrophages Reveals New Mechanisms of Extracellular and Intracellular Survival.Infection and immunity · 2015Article
- Reprogramming of Yersinia from virulent to persistent mode revealed by complex in vivo RNA-seq analysis.PLoS pathogens · 2015Article
- New insights into how Yersinia pestis adapts to its mammalian host during bubonic plague.PLoS pathogens · 2014Article
- Role of Yersinia pestis toxin complex family proteins in resistance to phagocytosis by polymorphonuclear leukocytes.Infection and immunity · 2013Article
- Yersinia pestis: mechanisms of entry into and resistance to the host cell.Frontiers in cellular and infection microbiology · 2013Review
- Studying Salmonellae and Yersiniae host-pathogen interactions using integrated 'omics and modeling.Current topics in microbiology and immunology · 2013Review
- Host-Brucella interactions and the Brucella genome as tools for subunit antigen discovery and immunization against brucellosis.Frontiers in cellular and infection microbiology · 2013Review
- Yersinia pestis insecticidal-like toxin complex (Tc) family proteins: characterization of expression, subcellular localization, and potential role in infection of the flea vector.BMC microbiology · 2012Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Yersinia pestis, the causative agent of plague, is a facultative intracellular pathogen. Previous studies have indicated that the ability of Y. pestis to survive inside macrophages may be critical during the early stages of plague pathogenesis. To gain insights into the biology of intracellular Y. pestis and its environment following phagocytosis, we determined the genome-wide transcriptional profile of Y. pestis KIM5 replicating inside J774.1 macrophage-like cells using DNA microarrays. At 1.5, 4, and 8 h postinfection, a total of 801, 464, and 416 Y. pestis genes were differentially regulated, respectively, compared to the level of gene expression of control bacteria grown in tissue culture medium. A number of stress-response genes, including those involved in detoxification of reactive oxygen species, as well as several metabolic genes involved in macromolecule synthesis, were significantly induced in intracellular Y. pestis, consistent with the presence of oxidative stress and nutrient starvation inside Yersinia-containing vacuoles. A putative stress-induced operon consisting of y2313, y2315, and y2316 (y2313-y2316), and a previously unidentified open reading frame, orfX, was studied further on the basis of its high level of intracellular expression. Mutant strains harboring either deletion, Deltay2313-y2316 or DeltaorfX, exhibited diverse phenotypes, including reduced effector secretion by the type III secretion system, increased intracellular replication, and filamentous morphology of the bacteria growing inside macrophages. The results suggest a possible role for these genes in regulating cell envelope characteristics in the intracellular environment.
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