ArticleGenome biology2010
Active DNA demethylation in human postmitotic cells correlates with activating histone modifications, but not transcription levels.
Article in Genome biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 91 citations in OpenAlex.
- Epigenetic regulation of innate immune dynamics during inflammation.Journal of leukocyte biology · 2024Review
- Lactate: a rising star in tumors and inflammation.Frontiers in immunology · 2024Review
- Postmitotic differentiation of human monocytes requires cohesin-structured chromatin.Nature communications · 2022Article
- Patterns of Maternal Neutrophil Gene Expression at 30 Weeks of Gestation, but Not DNA Methylation, Distinguish Mild from Severe Preeclampsia.International journal of molecular sciences · 2021Article
- The Road to Low-Dose Aspirin Therapy for the Prevention of Preeclampsia Began with the Placenta.International journal of molecular sciences · 2021Review
- The epigenetic pioneer EGR2 initiates DNA demethylation in differentiating monocytes at both stable and transient binding sites.Nature communications · 2021Article
- Role of Methylation in Pro- and Anti-Cancer Immunity.Cancers · 2021Review
- DNA Methylation as a Marker of Body Shape in Premenopausal Women.Frontiers in genetics · 2021Article
- Exposome and Immunity Training: How Pathogen Exposure Order Influences Innate Immune Cell Lineage Commitment and Function.International journal of molecular sciences · 2020Review
- Proteases Activate Pregnancy Neutrophils by a Protease-Activated Receptor 1 Pathway: Epigenetic Implications for Preeclampsia.Reproductive sciences (Thousand Oaks, Calif.) · 2020Article
- Protease Amplification of the Inflammatory Response Induced by Commensal Bacteria: Implications for Racial Disparity in Term and Preterm Birth.Reproductive sciences (Thousand Oaks, Calif.) · 2020Article
- TET Enzymes and 5hmC in Adaptive and Innate Immune Systems.Frontiers in immunology · 2019Review
- Tissue-specific DNA methylation loss during ageing and carcinogenesis is linked to chromosome structure, replication timing and cell division rates.Nucleic acids research · 2018Article
- TET2- and TDG-mediated changes are required for the acquisition of distinct histone modifications in divergent terminal differentiation of myeloid cells.Nucleic acids research · 2017Article
- Controlled re-activation of epigenetically silenced Tet promoter-driven transgene expression by targeted demethylation.Nucleic acids research · 2017Article
- Pulmonary endothelial cell DNA methylation signature in pulmonary arterial hypertension.Oncotarget · 2017Article
- DNA methylation: an epigenetic mark of cellular memory.Experimental & molecular medicine · 2017Review
- DNA Methylation and the Potential Role of Methyl-Containing Nutrients in Cardiovascular Diseases.Oxidative medicine and cellular longevity · 2017Review
- Alu repeats as transcriptional regulatory platforms in macrophage responses to M. tuberculosis infection.Nucleic acids research · 2016Article
- Activation-induced cytidine deaminase selectively catalyzed active DNA demethylation in pluripotency gene and improved cell reprogramming in bovine SCNT embryo.Cytotechnology · 2016Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn mammals, the dynamics of DNA methylation, in particular the regulated, active removal of cytosine methylation, has remained a mystery, partly due to the lack of appropriate model systems to study DNA demethylation. Previous work has largely focused on proliferating cell types that are mitotically arrested using pharmacological inhibitors to distinguish between active and passive mechanisms of DNA demethylation.
resultsWe explored this epigenetic phenomenon in a natural setting of post-mitotic cells: the differentiation of human peripheral blood monocytes into macrophages or dendritic cells, which proceeds without cell division. Using a global, comparative CpG methylation profiling approach, we identified many novel examples of active DNA demethylation and characterized accompanying transcriptional and epigenetic events at these sites during monocytic differentiation. We show that active DNA demethylation is not restricted to proximal promoters and that the time-course of demethylation varies for individual CpGs. Irrespective of their location, the removal of methylated cytosines always coincided with the appearance of activating histone marks.
conclusionsDemethylation events are highly reproducible in monocyte-derived dendritic cells from different individuals. Our data suggest that active DNA demethylation is a precisely targeted event that parallels or follows the modification of histones, but is not necessarily coupled to alterations in transcriptional activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.