ArticleAnalytical chemistry2010
Multiplexed detection and characterization of rare tumor cells in Hodgkin's lymphoma with multicolor quantum dots.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 108 citations in OpenAlex.
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- Integration of isothermal amplification with quantum dot-based fluorescence resonance energy transfer for simultaneous detection of multiple microRNAs.Chemical science · 2018Article
- Multiplexed Optical Imaging of Tumor-Directed Nanoparticles: A Review of Imaging Systems and Approaches.Nanotheranostics · 2017Review
- Biomedical Probes Based on Inorganic Nanoparticles for Electrochemical and Optical Spectroscopy Applications.Sensors (Basel, Switzerland) · 2015Review
- Single-band upconversion nanoprobes for multiplexed simultaneous in situ molecular mapping of cancer biomarkers.Nature communications · 2015Article
- Quantum dots for quantitative imaging: from single molecules to tissue.Cell and tissue research · 2015Review
- Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats.International journal of nanomedicine · 2015Article
- Post-genomics nanotechnology is gaining momentum: nanoproteomics and applications in life sciences.Omics : a journal of integrative biology · 2014Review
- Revisiting 30 years of biofunctionalization and surface chemistry of inorganic nanoparticles for nanomedicine.Frontiers in chemistry · 2014Review
- Multicolor multicycle molecular profiling with quantum dots for single-cell analysis.Nature protocols · 2013Article
- Nanoparticles for Improving Cancer Diagnosis.Materials science & engineering. R, Reports : a review journal · 2013Article
- Nanocrystal Core Lipoprotein Biomimetics for Imaging of Lipoproteins and Associated Diseases.Current cardiovascular imaging reports · 2013Article
- Semiconductor quantum dots for bioimaging and biodiagnostic applications.Annual review of analytical chemistry (Palo Alto, Calif.) · 2013Review
- Quantum dot imaging platform for single-cell molecular profiling.Nature communications · 2013Article
- Quantum dots: synthesis, bioapplications, and toxicity.Nanoscale research letters · 2012Article
- Spectral unmixing imaging of wavelength-responsive fluorescent probes: an application for the real-time report of amyloid Beta species in Alzheimer's disease.Molecular imaging and biology · 2012Article
- Sensitive and rapid quantification of C-reactive protein using quantum dot-labeled microplate immunoassay.Journal of translational medicine · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The multicolor and multiplexing capabilities of semiconductor quantum dots (QDs) are most promising for improving the sensitivity and specificity of in vitro molecular and cellular diagnostics. Here, we report the use of multiplexed QDs and wavelength-resolved imaging to detect and characterize a class of low-abundant tumor cells in Hodgkin's lymphoma. Known as the Hodgkin's and Reed-Sternberg (HRS) cells, this class of malignant cells is a pathological hallmark in clinical diagnosis, but it comprises only about 1% of the heterogeneous infiltrating cells in lymph node tissues. To overcome this cellular heterogeneity and rarity problem, we have developed multicolor QD-antibody conjugates to simultaneously detect a panel of four protein biomarkers (CD15, CD30, CD45, and Pax5) directly on human tissue biopsies. This multiplexing approach allows rapid detection and differentiation of rare HRS cells from infiltrating immune cells such as T and B lymphocytes. We have also carried out clinical translation studies involving six confirmed Hodgkin's lymphoma patients, two suspicious lymphoma cases, and two patients with reactive lymph nodes (but not lymphoma). The results indicate that a distinct QD staining pattern (CD15 positive, CD30 positive, CD45 negative, and Pax5 positive) can be used to not only detect Hodgkin's lymphoma but also differentiate it from benign lymphoid hyperplasia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.