ArticleMolecular biology reports2011
Unraveling new genes associated with seed development and metabolism in Bixa orellana L. by expressed sequence tag (EST) analysis.
Article in Molecular biology reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 35 citations in OpenAlex.
- Evolutionary adaptation of CCD4 enzymes inFrontiers in plant science · 2026Article
- Advances in Tissue Culture and Transformation Studies in Non-model Species: Bixa orellana L. (Bixaceae).Methods in molecular biology (Clifton, N.J.) · 2024Article
- Dynamics of annatto pigment synthesis and accumulation in seeds of Bixa orellana L. revealed by integrated chemical, anatomical, and RNA-Seq analyses.Protoplasma · 2023Article
- IdentifyingFrontiers in plant science · 2022Article
- Genome-Wide Identification and Expression Analysis of AP2/ERF Transcription Factor Related to Drought Stress in Cultivated Peanut (Frontiers in genetics · 2021Article
- The biotechnology (genetic transformation and molecular biology) of Bixa orellana L. (achiote).Planta · 2018Review
- Selection and validation of reference genes for quantitative gene expression analyses in various tissues and seeds at different developmental stages inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Carotenoid Cleavage Oxygenases from Microbes and Photosynthetic Organisms: Features and Functions.International journal of molecular sciences · 2016Review
- De novo transcriptome sequencing in Bixa orellana to identify genes involved in methylerythritol phosphate, carotenoid and bixin biosynthesis.BMC genomics · 2015Article
- New target carotenoids for CCD4 enzymes are revealed with the characterization of a novel stress-induced carotenoid cleavage dioxygenase gene from Crocus sativus.Plant molecular biology · 2014Article
- Functional diversity of 2-oxoglutarate/Fe(II)-dependent dioxygenases in plant metabolism.Frontiers in plant science · 2014Review
- MAP65-1a positively regulates H2O2 amplification and enhances brassinosteroid-induced antioxidant defence in maize.Journal of experimental botany · 2013Article
- Development of ESTs and data mining of pineapple EST-SSRs.Molecular biology reports · 2012Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tropical tree Bixa orellana L. produces a range of secondary metabolites which biochemical and molecular biosynthesis basis are not well understood. In this work we have characterized a set of ESTs from a non-normalized cDNA library of B. orellana seeds to obtain information about the main developmental and metabolic processes taking place in developing seeds and their associated genes. After sequencing a set of randomly selected clones, most of the sequences were assigned with putative functions based on similarity, GO annotations and protein domains. The most abundant transcripts encoded proteins associated with cell wall (prolyl 4-hydroxylase), fatty acid (acyl carrier protein), and hormone/flavonoid (2OG-Fe oxygenase) synthesis, germination (MADS FLC-like protein) and embryo development (AP2/ERF transcription factor) regulation, photosynthesis (chlorophyll a-b binding protein), cell elongation (MAP65-1a), and stress responses (metallothionein- and thaumatin-like proteins). Enzymes were assigned to 16 different metabolic pathways related to both primary and secondary metabolisms. Characterization of two candidate genes of the bixin biosynthetic pathway, BoCCD and BoOMT, showed that they belong, respectively, to the carotenoid-cleavage dioxygenase 4 (CCD4) and caffeic acid O-methyltransferase (COMT) families, and are up-regulated during seed development. It indicates their involvement in the synthesis of this commercially important carotenoid pigment in seeds of B. orellana. Most of the genes identified here are the first representatives of their gene families in B. orellana.
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