ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2010
Evaluation of seed storage-protein gene 5' untranslated regions in enhancing gene expression in transgenic rice seed.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 30 citations in OpenAlex.
- Engineering non-coding DNA Elements in Rice: an Elegant Approach To fine-tune Agronomical Advantageous Traits.Rice (New York, N.Y.) · 2025Review
- Deciphering the Contribution of TATA Box and 5'UTR to Defense Signaling in Rice Under Blast Infection.Biology · 2025Review
- Multi-Omics Analysis Reveals ThatFrontiers in plant science · 2022Article
- Glutelin subtype-dependent protein localization in rice grain evidenced by immunodetection analyses.Plant molecular biology · 2019Article
- Improved expression of porcine epidemic diarrhea antigen by fusion with cholera toxin B subunit and chloroplast transformation inPlant cell, tissue and organ culture · 2019Article
- TF2Network: predicting transcription factor regulators and gene regulatory networks in Arabidopsis using publicly available binding site information.Nucleic acids research · 2018Article
- A Collection of Conserved Noncoding Sequences to Study Gene Regulation in Flowering Plants.Plant physiology · 2016Article
- Plant exomics: concepts, applications and methodologies in crop improvement.Plant signaling & behavior · 2015Review
- Inference of transcriptional networks in Arabidopsis through conserved noncoding sequence analysis.The Plant cell · 2014Article
- The promoter and the 5'-untranslated region of rice metallothionein OsMT2b gene are capable of directing high-level gene expression in germinated rice embryos.Plant cell reports · 2014Article
- Article
- Truncated cotton subtilase promoter directs guard cell-specific expression of foreign genes in tobacco and Arabidopsis.PloS one · 2013Article
- Conserved noncoding sequences highlight shared components of regulatory networks in dicotyledonous plants.The Plant cell · 2012Article
- Evaluation of seed storage protein gene 3'-untranslated regions in enhancing gene expression in transgenic rice seed.Transgenic research · 2012Article
- Identification and evaluation of ω-3 fatty acid desaturase genes for hyperfortifying α-linolenic acid in transgenic rice seed.Journal of experimental botany · 2012Article
- Evolutionary divergence and limits of conserved non-coding sequence detection in plant genomes.Nucleic acids research · 2011Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
5' untranslated regions (UTRs) are important sequence elements that modulate the expression of genes. Using the β-glucuronidase (GUS) reporter gene driven by the GluC promoter for the rice-seed storage-protein glutelin, we evaluated the potential of the 5'-UTRs of six seed storage-protein genes in enhancing the expression levels of the foreign gene in stable transgenic rice lines. All of the 5'-UTRs significantly enhanced the expression level of the GluC promoter without altering its expression pattern. The 5'-UTRs of Glb-1 and GluA-1 increased the expression of GUS by about 3.36- and 3.11-fold, respectively. The two 5'-UTRs downstream of the Glb-1, OsAct2 and CMV35S promoters also increased GUS expression level in stable transgenic rice lines or in transient expression protoplasts. Therefore, the enhancements were independent of the promoter sequence. Real-time quantitative RT-PCR analysis showed that the increase in protein production was not accompanied by alteration in mRNA levels, which suggests that the enhancements were due to increasing the translational efficiencies of the mRNA. The 5'-UTRs of Glb-1 and GluA-1, when combined with strong promoters, might be ideal candidates for high production of recombinant proteins in rice seeds.
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