ArticleThe EMBO journal2010
Opposing functions of two sub-domains of the SNARE-complex in neurotransmission.
Article in The EMBO journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 60 citations in OpenAlex.
- SNAP-25 disease variants affect synaptic transmission by destabilizing SNARE complexes within a multimeric SNARE ring.Cell reports · 2026Article
- Multiple structural states in an intrinsically disordered protein, SNAP-25, using circular dichroism.Biophysical journal · 2025Article
- Mechanisms that regulate the C1-C2B mutual inhibition control functional switch of UNC-13.eLife · 2025Article
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- Neuromuscular recovery from botulism involves multiple forms of compensatory plasticity.Frontiers in cellular neuroscience · 2023Article
- A Hyperactive Form of unc-13 Enhances CaCell reports · 2019Article
- Vti1a/b regulate synaptic vesicle and dense core vesicle secretion via protein sorting at the Golgi.Nature communications · 2018Article
- Munc13-1 and Munc18-1 together prevent NSF-dependent de-priming of synaptic vesicles.Nature communications · 2017Article
- Interactions Between SNAP-25 and Synaptotagmin-1 Are Involved in Vesicle Priming, Clamping Spontaneous and Stimulating Evoked Neurotransmission.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Kinetic barriers to SNAREpin assembly in the regulation of membrane docking/priming and fusion.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Mechanisms, pools, and sites of spontaneous vesicle release at synapses of rod and cone photoreceptors.The European journal of neuroscience · 2016Article
- Presynaptic inhibition upon CB1 or mGlu2/3 receptor activation requires ERK/MAPK phosphorylation of Munc18-1.The EMBO journal · 2016Article
- Function Suggests Nano-Structure: Quantitative Structural Support for SNARE-Mediated Pore Formation.Neurotoxicity research · 2016Article
- Competition and Homeostasis of Excitatory and Inhibitory Connectivity in the Adult Mouse Visual Cortex.Cerebral cortex (New York, N.Y. : 1991) · 2015Article
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- Molecular underpinnings of synaptic vesicle pool heterogeneity.Traffic (Copenhagen, Denmark) · 2015Review
- The SNARE protein vti1a functions in dense-core vesicle biogenesis.The EMBO journal · 2014Article
- A half-zippered SNARE complex represents a functional intermediate in membrane fusion.Journal of the American Chemical Society · 2014Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The SNARE-complex consisting of synaptobrevin-2/VAMP-2, SNAP-25 and syntaxin-1 is essential for evoked neurotransmission and also involved in spontaneous release. Here, we used cultured autaptic hippocampal neurons from Snap-25 null mice rescued with mutants challenging the C-terminal, N-terminal and middle domains of the SNARE-bundle to dissect out the involvement of these domains in neurotransmission. We report that the stabilities of two different sub-domains of the SNARE-bundle have opposing functions in setting the probability for both spontaneous and evoked neurotransmission. Destabilizing the C-terminal end of the SNARE-bundle abolishes spontaneous neurotransmitter release and reduces evoked release probability, indicating that the C-terminal end promotes both modes of release. In contrast, destabilizing the middle or deleting the N-terminal end of the SNARE-bundle increases both spontaneous and evoked release probabilities. In both cases, spontaneous release was affected more than evoked neurotransmission. In addition, the N-terminal deletion delays vesicle priming after a high-frequency train. We propose that the stability of N-terminal two-thirds of the SNARE-bundle has a function for vesicle priming and limiting spontaneous release.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.