ArticleJournal of bacteriology2010
The LysR-type virulence activator AphB regulates the expression of genes in Vibrio cholerae in response to low pH and anaerobiosis.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed.
- The crosstalk between acute hepatopancreatic necrosis disease (AHPND) and the Vibrio quorum sensing (QS) system: A review.PLoS pathogens · 2026Review
- AdiY acts as a cytoplasmic pH sensor via histidine protonation to regulate acid stress adaptation inJournal of bacteriology · 2026Article
- Fundamentals and Exceptions of the LysR-type Transcriptional Regulators.ACS synthetic biology · 2024Review
- Article
- An In Vitro System Mimics the Intestinal Microbiota of Striped Beakfish (Animals : an open access journal from MDPI · 2024Article
- Non-O1/Non-O139Microorganisms · 2024Review
- L-Ascorbic Acid RestrictsMicroorganisms · 2024Article
- Magnetic chitin beads (MCB) coated withGut microbes · 2023Article
- A bacterial binary toxin system that kills both insects and aquatic crustaceans: Photorhabdus insect-related toxins A and B.PLoS pathogens · 2023Review
- The Interplay of AphB and CadC to Activate Acid Resistance ofJournal of bacteriology · 2023Article
- Stress Responses in Pathogenic Vibrios and Their Role in Host and Environmental Survival.Advances in experimental medicine and biology · 2023Article
- Advances in cholera research: from molecular biology to public health initiatives.Frontiers in microbiology · 2023Review
- Structural Insights into Regulation of Vibrio Virulence Gene Networks.Advances in experimental medicine and biology · 2023Article
- The pH Robustness of Bacterial Sensing.mBio · 2022Article
- Vibrio cholerae senses human enteric α-defensin 5 through a CarSR two-component system to promote bacterial pathogenicity.Communications biology · 2022Article
- S-Nitrosylation of the virulence regulator AphB promotes Vibrio cholerae pathogenesis.PLoS pathogens · 2022Article
- ToxT Regulon Is Nonessential for Vibrio cholerae Colonization in Adult Mice.Applied and environmental microbiology · 2022Article
- Expression of the AHPND Toxins PirAInternational journal of molecular sciences · 2022Article
- Prediction and Inferred Evolution of Acid Tolerance Genes in the Biotechnologically ImportantFrontiers in microbiology · 2022Article
- Bile Salts Promote ToxR Regulon Activation during Growth under Virulence-Inducing Conditions.Infection and immunity · 2021Article
17 more citing papers are in PubMed but not listed here.
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3 authors.
Funding
Abstract
AphB is a LysR-type activator that initiates the expression of the virulence cascade in Vibrio cholerae by cooperating with the quorum-sensing-regulated activator AphA at the tcpPH promoter on the Vibrio pathogenicity island (VPI). To identify the ancestral chromosomal genes in V. cholerae regulated by AphB, we carried out a microarray analysis and show here that AphB influences the expression of a number of genes that are not associated with the VPI. One gene strongly activated by AphB is cadC, which encodes the ToxR-like transcriptional activator responsible for activating the expression of lysine decarboxylase, which plays an important role in survival at low pH. Other genes activated by AphB encode a Na(+)/H(+) antiporter, a carbonic anhydrase, a member of the ClC family of chloride channels, and a member of the Gpr1/Fun34/YaaH family. AphB influences each of these genes directly by recognizing a conserved binding site within their promoters, as determined by gel mobility shift assays. Transcriptional lacZ fusions indicate that AphB activates the expression of these genes under aerobic conditions in response to low pH and also under anaerobic conditions at neutral pH. Further experiments show that the regulation of cadC by AphB in response to low pH and anaerobiosis is mirrored in the heterologous organism Escherichia coli, is independent of the global regulators Fnr and ArcAB, and depends upon the region of the promoter that contains the AphB binding site. These results raise the possibility that the activity of AphB is influenced by the pH and oxygen tension of the environment.
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