ArticleApplied and environmental microbiology2010
Influence of temperature and predation on survival of Salmonella enterica serovar Typhimurium and expression of invA in soil and manure-amended soil.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Article
- Prediction of edapho-climatic parameters in the incidence of Campylobacter spp. in northwestern Mexico.PLOS global public health · 2024Article
- Advances in detection methods for viable Salmonella spp.: current applications and challenges.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Review
- The Fate of Foodborne Pathogens in Manure Treated Soil.Frontiers in microbiology · 2021Review
- Development of PMAxxFrontiers in microbiology · 2020Article
- Article
- Article
- A Rapid Method for Detection ofFrontiers in microbiology · 2019Article
- Sources and contamination routes of microbial pathogens to fresh produce during field cultivation: A review.Food microbiology · 2018Review
- Characterization of biofilm formation by Salmonella enterica at the air-liquid interface in aquatic environments.Environmental monitoring and assessment · 2018Article
- Regulatory Issues Associated with Preharvest Food Safety: United States Perspective.Microbiology spectrum · 2017Review
- Article
- Fate of Viable but Non-culturable Listeria monocytogenes in Pig Manure Microcosms.Frontiers in microbiology · 2016Article
- Persistence and Potential Viable but Non-culturable State of Pathogenic Bacteria during Storage of Digestates from Agricultural Biogas Plants.Frontiers in microbiology · 2016Article
- Experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents.BMC microbiology · 2015Article
- Ecological prevalence, genetic diversity, and epidemiological aspects of Salmonella isolated from tomato agricultural regions of the Virginia Eastern Shore.Frontiers in microbiology · 2015Article
- Protozoan predation is differentially affected by motility of enteric pathogens in water vs. sediments.Microbial ecology · 2014Article
- Enteric pathogen-plant interactions: molecular connections leading to colonization and growth and implications for food safety.Microbes and environments · 2014Review
- Draft Genome Sequence of Salmonella enterica subsp. enterica Serotype Oranienburg Strain S-76, Isolated from an Aquatic Environment.Genome announcements · 2013Article
- Colonization and internalization of Salmonella enterica in tomato plants.Applied and environmental microbiology · 2013Article
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5 authors.
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Abstract
The effects of three temperatures (5, 15, and 25 degrees C) on the survival of Salmonella enterica serovar Typhimurium in topsoil were investigated in small microcosms by three different techniques: plate counting, invA gene quantification, and invA mRNA quantification. Differences in survival were related to the effect of protozoan predation. Tetracycline-resistant Salmonella serovar Typhimurium was inoculated into soil and manure-amended soil at 1.5 x 10(8) cells g soil(-1). Population densities were determined by plate counting and by molecular methods and monitored for 42 days. Simultaneous extraction of RNA and DNA, followed by quantitative PCR, was used to investigate invA gene levels and expression. Analysis by these three techniques showed that Salmonella serovar Typhimurium survived better at 5 degrees C. Comparing DNA and CFU levels, significantly higher values were determined by DNA-based techniques. invA mRNA levels showed a fast decrease in activity, with no detectable mRNA after an incubation period of less than 4 days in any of the soil scenarios. A negative correlation was found between Salmonella serovar Typhimurium CFU levels and protozoan most probable numbers, and we propose the role of the predator-prey interaction as a factor to explain the die-off of the introduced strain by both culture- and DNA quantification-based methods. The results indicate that temperature, manure, and protozoan predation are important factors influencing the survival of Salmonella serovar Typhimurium in soil.
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