ArticleApplied and environmental microbiology2010
Novel high-throughput detection method to assess bacterial surfactant production.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 109 citations in OpenAlex.
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- Mucin-induced surface dispersal ofmBio · 2024Article
- Surface-active antibiotic production as a multifunctional adaptation for postfire microorganisms.The ISME journal · 2024Article
- High-throughput evaluation of hemolytic activity through precise measurement of colony and hemolytic zone sizes of engineeredBiology methods & protocols · 2024Article
- Article
- Exopolysaccharides amylovoran and levan contribute to sliding motility in the fire blight pathogen Erwinia amylovora.Environmental microbiology · 2022Article
- Throughput screening of Bacillus subtilis strains that abundantly secrete surfactin in vitro identifies effective probiotic candidates.PloS one · 2022Article
- Screening Strategies for Biosurfactant Discovery.Advances in biochemical engineering/biotechnology · 2022Article
- Exploring the expression and functionality of theRNA biology · 2021Article
- Biosurfactants Produced by Phyllosphere-Colonizing Pseudomonads Impact Diesel Degradation but Not Colonization of Leaves of Gnotobiotic Arabidopsis thaliana.Applied and environmental microbiology · 2021Article
- The RsmA RNA-Binding Proteins inFrontiers in plant science · 2021Article
- Microbial biosurfactant research: time to improve the rigour in the reporting of synthesis, functional characterization and process development.Microbial biotechnology · 2021Review
- Emerging priorities for microbial metagenome research.Bioresource technology reports · 2020Review
- Production and characterization of surfactin-like biosurfactant produced by novel strain Bacillus nealsonii S2MT and it's potential for oil contaminated soil remediation.Microbial cell factories · 2020Article
- Biosurfactant Production in Sub-Oxic Conditions Detected in Hydrocarbon-Degrading Isolates from Marine and Estuarine Sediments.International journal of environmental research and public health · 2020Article
- A Straightforward Assay for Screening and Quantification of Biosurfactants in Microbial Culture Supernatants.Frontiers in bioengineering and biotechnology · 2020Article
- Quorum Sensing Controls Both Rhamnolipid and Polyhydroxyalkanoate Production inFrontiers in bioengineering and biotechnology · 2020Article
- Re-programming of Pseudomonas syringae pv. actinidiae gene expression during early stages of infection of kiwifruit.BMC genomics · 2018Article
- Novel Method Reveals a Narrow Phylogenetic Distribution of Bacterial Dispersers in Environmental Communities Exposed to Low-Hydration Conditions.Applied and environmental microbiology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel biosurfactant detection assay was developed for the observation of surfactants on agar plates. By using an airbrush to apply a fine mist of oil droplets, surfactants can be observed instantaneously as halos around biosurfactant-producing colonies. This atomized oil assay can detect a wide range of different synthetic and bacterially produced surfactants. This method could detect much lower concentrations of many surfactants than a commonly used water drop collapse method. It is semiquantitative and therefore has broad applicability for uses such as high-throughput mutagenesis screens of biosurfactant-producing bacterial strains. The atomized oil assay was used to screen for mutants of the plant pathogen Pseudomonas syringae pv. syringae B728a that were altered in the production of biosurfactants. Transposon mutants displaying significantly altered surfactant halos were identified and further analyzed. All mutants identified displayed altered swarming motility, as would be expected of surfactant mutants. Additionally, measurements of the transcription of the syringafactin biosynthetic cluster in the mutants, the principal biosurfactant known to be produced by B728a, revealed novel regulators of this pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.