ArticleApplied and environmental microbiology2010
Metatranscriptomic analysis of the response of river biofilms to pharmaceutical products, using anonymous DNA microarrays.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 52 citations in OpenAlex.
- Pharmaceutical impacts on aerobic granular sludge morphology and potential implications for abiotic removal.Chemosphere · 2024Article
- Emergence of phenotypic and genotypic resistance in the intestinal microbiota of rainbow trout (Oncorhynchus mykiss) exposed long-term to sub-inhibitory concentrations of sulfamethoxazole.Ecotoxicology (London, England) · 2021Article
- The use of non-target high-resolution mass spectrometry screening to detect the presence of antibiotic residues in urban streams of Greensboro North Carolina.Journal of environmental health science & engineering · 2021Article
- Contrasting Effects of Environmental Concentrations of Sulfonamides on Microbial Heterotrophic Activities in Freshwater Sediments.Frontiers in microbiology · 2021Article
- Environmental Concentrations of Sulfonamides Can Alter Bacterial Structure and Induce Diatom Deformities in Freshwater Biofilm Communities.Frontiers in microbiology · 2021Article
- Article
- Review
- Effects of triclosan on bacterial community composition andElementa (Washington, D.C.) · 2017Article
- Article
- Review of Antimicrobial Resistance in the Environment and Its Relevance to Environmental Regulators.Frontiers in microbiology · 2016Review
- Microbial Community Composition, Functions, and Activities in the Gulf of Mexico 1 Year after the Deepwater Horizon Accident.Applied and environmental microbiology · 2015Article
- Integrated metagenomics and network analysis of soil microbial community of the forest timberline.Scientific reports · 2015Article
- CoMeta: classification of metagenomes using k-mers.PloS one · 2015Article
- Removal of pharmaceuticals and personal care products during water recycling: microbial community structure and effects of substrate concentration.Applied and environmental microbiology · 2014Article
- Minimum information about a biofilm experiment (MIABiE): standards for reporting experiments and data on sessile microbial communities living at interfaces.Pathogens and disease · 2014Review
- Aerobic biofilms grown from Athabasca watershed sediments are inhibited by increasing concentrations of bituminous compounds.Applied and environmental microbiology · 2013Article
- Human health implications of clinically relevant bacteria in wastewater habitats.Environmental science and pollution research international · 2013Review
- Next-generation sequencing of microbial communities in the Athabasca River and its tributaries in relation to oil sands mining activities.Applied and environmental microbiology · 2012Article
- Article
- Metagenomic analysis of the bioremediation of diesel-contaminated Canadian high arctic soils.PloS one · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pharmaceutical products are released at low concentrations into aquatic environments following domestic wastewater treatment. Such low concentrations have been shown to induce transcriptional responses in microorganisms, which could have consequences on aquatic ecosystem dynamics. In order to test if these transcriptional responses could also be observed in complex river microbial communities, biofilm reactors were inoculated with water from two rivers of differing trophic statuses and subsequently treated with environmentally relevant doses (ng/liter to microg/liter range) of four pharmaceuticals (erythromycin [ER], gemfibrozil [GM], sulfamethazine [SN], and sulfamethoxazole [SL]). To monitor functional gene expression, we constructed a 9,600-feature anonymous DNA microarray platform onto which cDNA from the biofilms was hybridized. Pharmaceutical treatments induced both positive and negative transcriptional responses from biofilm microorganisms. For instance, ER induced the transcription of several stress, transcription, and replication genes, while GM, a lipid regulator, induced transcriptional responses from several genes involved in lipid metabolism. SN caused shifts in genes involved in energy production and conversion, and SL induced responses from a range of cell membrane and outer envelope genes, which in turn could affect biofilm formation. The results presented here demonstrate for the first time that low concentrations of small molecules can induce transcriptional changes in a complex microbial community. The relevance of these results also demonstrates the usefulness of anonymous DNA microarrays for large-scale metatranscriptomic studies of communities from differing aquatic ecosystems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.