ArticleEnvironmental health perspectives2010
Low doses of the carcinogen furan alter cell cycle and apoptosis gene expression in rat liver independent of DNA methylation.
Article in Environmental health perspectives, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 42 citations in OpenAlex.
- Assessment of the oxidative damage and apoptotic pathway related to furan cytotoxicity in cultured mouse Leydig cells.Toxicology research · 2023Article
- Article
- Exposure assessment of process-related contaminants in food by biomarker monitoring.Archives of toxicology · 2018Review
- Risks for public health related to the presence of furan and methylfurans in food.EFSA journal. European Food Safety Authority · 2017Article
- Low dose assessment of the carcinogenicity of furan in male F344/N Nctr rats in a 2-year gavage study.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2017Article
- Toxicogenomic assessment of liver responses following subchronic exposure to furan in Fischer F344 rats.Archives of toxicology · 2016Article
- Persistence of furan-induced epigenetic aberrations in the livers of F344 rats.Toxicological sciences : an official journal of the Society of Toxicology · 2015Article
- Dose- and time-dependent epigenetic changes in the livers of Fisher 344 rats exposed to furan.Toxicological sciences : an official journal of the Society of Toxicology · 2014Article
- Trichloroethylene-induced gene expression and DNA methylation changes in B6C3F1 mouse liver.PloS one · 2014Article
- Reactive metabolites in the biotransformation of molecules containing a furan ring.Chemical research in toxicology · 2013Review
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- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEvidence of potent rodent carcinogenicity via an unclear mechanism suggests that furan in various foods [leading to an intake of up to 3.5 microg/kg body weight (bw)/day] may present a potential risk to human health.
objectivesWe tested the hypothesis that altered expression of genes related to cell cycle control, apoptosis, and DNA damage may contribute to the carcinogenicity of furan in rodents. In addition, we investigated the reversibility of such changes and the potential role of epigenetic mechanisms in response to furan doses that approach the maximum estimated dietary intake in humans.
methodsThe mRNA expression profiles of genes related to cell cycle, apoptosis, and DNA damage in rat liver treated with furan concentrations of 0.1 and 2 mg/kg bw were measured by quantitative polymerase chain reaction (PCR) arrays. We assessed epigenetic changes by analysis of global and gene-specific DNA methylation [methylation-specific PCR, combined bisulfite restriction analysis (COBRA), and methylated DNA immunoprecipitation chip] and microRNA (miRNA) analyses.
resultsThe expression profiles of apoptosis-related and cell-cycle-related genes were unchanged after 5 days of treatment, although we observed a statistically significant change in the expression of genes related to cell cycle control and apoptosis, but not DNA damage, after 4 weeks of treatment. These changes were reversed after an off-dose period of 2 weeks. None of the gene expression changes was associated with a change in DNA methylation, although we detected minor changes in the miRNA expression profile (5 miRNA alterations out of 349 measured) that may have contributed to modification of gene expression in some cases.
conclusionNongenotoxic changes in gene expression may contribute to the carcinogenicity of furan in rodents. These findings highlight the need for a more comprehensive risk assessment of furan exposure in humans.
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