ArticleThe Journal of clinical investigation1991
Treatment with recombinant human tumor necrosis factor-alpha protects rats against the lethality, hypotension, and hypothermia of gram-negative sepsis.
Article in The Journal of clinical investigation, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 159 citations in OpenAlex.
- Jeffrey Norton and the Legacy of the Surgical Metabolism Section of the Surgery Branch of the NCI.Annals of surgical oncology · 2024Article
- A Comprehensive Safety Trial of Chimeric Antibody 14.18 With GM-CSF, IL-2, and Isotretinoin in High-Risk Neuroblastoma Patients Following Myeloablative Therapy: Children's Oncology Group Study ANBL0931.Frontiers in immunology · 2018Article
- Resuscitation fluid composition affects hepatic inflammation in a murine model of early sepsis.Intensive care medicine experimental · 2017Article
- TNF Tolerance in Monocytes and Macrophages: Characteristics and Molecular Mechanisms.Journal of immunology research · 2017Review
- Kavain Inhibition of LPS-Induced TNF-α via ERK/LITAF.Toxicology research · 2016Article
- A rat model of concurrent combined injuries (polytrauma).International journal of clinical and experimental medicine · 2015Article
- Hypometabolism and hypothermia in the rat model of endotoxic shock: independence of circulatory hypoxia.The Journal of physiology · 2014Article
- Inactivation of renal mitochondrial respiratory complexes and manganese superoxide dismutase during sepsis: mitochondria-targeted antioxidant mitigates injury.American journal of physiology. Renal physiology · 2014Article
- Effect of chronic ethanol consumption in female rats subjected to experimental sepsis.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2013Article
- Nociceptin receptor signaling in sympathetic neurons from septic rats.The Journal of surgical research · 2013Article
- How can antibiotics worsen acute kidney injury but improve survival in experimental sepsis?.Critical care medicine · 2012Article
- Chronic kidney disease worsens sepsis and sepsis-induced acute kidney injury by releasing High Mobility Group Box Protein-1.Kidney international · 2011Article
- Peritoneal damage: the inflammatory response and clinical implications of the neuro-immuno-humoral axis.World journal of surgery · 2010Review
- Animal models of sepsis and sepsis-induced kidney injury.The Journal of clinical investigation · 2009Review
- Staphylococcus aureus infection after splenectomy and splenic autotransplantation in BALB/c mice.Clinical and experimental immunology · 2008Article
- What We Do When We Suppress Fever.Current infectious disease reports · 2005Article
- Identification and functional characterization of a novel binding site on TNF-alpha promoter.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Resistance/susceptibility to Echinococcus multilocularis infection and cytokine profile in humans. II. Influence of the HLA B8, DR3, DQ2 haplotype.Clinical and experimental immunology · 2000Article
- Molecular and functional analysis of the human prothrombinase gene (HFGL2) and its role in viral hepatitis.The American journal of pathology · 2000Article
- Tumour necrosis factor gene polymorphisms as severity markers in rheumatoid arthritis.Annals of the rheumatic diseases · 1999Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tumor necrosis factor (TNF) is a peptide secreted by macrophages in response to endotoxin that can produce many of the changes seen in septic shock. After cecal ligation and puncture (CLP) rats gradually develop tachycardia, hypotension, tachypnea, and hypothermia. At 5 h post-CLP, rats have a peak in serum levels of endotoxin and 60% of rats have blood cultures that grow Gram-negative rods (Escherichia coli and Klebsiella pneumonia). At 20 h post-CLP all rats develop positive blood cultures. Serum levels of TNF are not reproducibly measurable in rats following CLP. Rats undergoing CLP have a 50-80% mortality with deaths usually occurring 24-72 h postinjury. Repetitive (twice daily x 6 d) i.p. injection of sublethal doses of recombinant human TNF-alpha (100 micrograms/kg) to rats undergoing CLP 1 d after the treatment period resulted in a significant reduction in mortality compared to control rats previously unexposed to rTNF (P less than 0.03). Animals treated with rTNF had no hypotension or hypothermia after CLP and regained normal food intake faster than control rats. 12 h after CLP the gene expression for manganous superoxide dismutase (MnSOD), an inducible mitochondrial metalloenzyme responsible for cellular resistance to injury from toxic reactive oxygen species, was higher in livers of rats treated with rTNF suggesting that the TNF treatment augmented expression of this protective enzyme. Unlike MnSOD, expression of the gene for copper-zinc SOD was not affected by CLP or rTNF treatment. The results suggest that prior treatment with recombinant TNF can ameliorate the lethality, hypotension, hypothermia, and anorexia of Gram-negative sepsis in rats and that the mechanism may be related to enhanced hepatic expression of the gene for MnSOD. Repeated administration of recombinant TNF may be a strategy to minimize mortality and morbidity of Gram-negative sepsis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.