ArticleAmerican journal of reproductive immunology (New York, N.Y. : 1989)2010
Leukocyte pyruvate kinase expression is reduced in normal human pregnancy but not in pre-eclampsia.
Article in American journal of reproductive immunology (New York, N.Y. : 1989), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 14 citations in OpenAlex.
- Immunometabolic adaptation and immune plasticity in pregnancy and the bi-directional effects of obesity.Clinical and experimental immunology · 2022Article
- Cellular immune responses in the pathophysiology of preeclampsia.Journal of leukocyte biology · 2022Review
- Does Altered Cellular Metabolism Underpin the Normal Changes to the Maternal Immune System during Pregnancy?Immunometabolism · 2021Article
- Article
- Bioenergetic analysis of human peripheral blood mononuclear cells.Clinical and experimental immunology · 2015Article
- New insights into the relationship between viral infection and pregnancy complications.American journal of reproductive immunology (New York, N.Y. : 1989) · 2014Article
- An enzymatic colorimetric assay for glucose-6-phosphate.Analytical biochemistry · 2011Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
problemEmerging evidence suggests that metabolism influences immune cell signaling and immunoregulation. To examine the immunoregulatory role of glycolysis in pregnancy, we evaluated the properties of pyruvate kinase in leukocytes from non-pregnant women and those with normal pregnancy and pre-eclampsia. METHOD OF STUDY: We evaluated pyruvate kinase expression in lymphocytes and neutrophils from non-pregnant, pregnant, and pre-eclampsia patients using fluorescence microscopy and flow cytometry. Leukocyte pyruvate kinase activity and pyruvate concentrations were also evaluated. To study pyruvate's effect on signaling, we labeled Jurkat T cells with Ca(2+) dyes and measured cell responses in the presence of agents influencing intracellular pyruvate.
resultsThe expression of pyruvate kinase is reduced in lymphocytes and neutrophils from normal pregnant women in comparison with those of non-pregnant women and pre-eclampsia patients. Similarly, the activity of pyruvate kinase and the intracellular pyruvate concentration are reduced in leukocytes of normal pregnant women in comparison with non-pregnant women and women with pre-eclampsia. Using Jurkat cells as a model of leukocyte signaling, we have shown that perturbations of intracellular pyruvate influence Ca(2+) signals.
conclusionNormal pregnancy is characterized by reduced pyruvate kinase expression within lymphocytes and neutrophils. We speculate that reduced pyruvate kinase expression modifies immune cell responses due to reduced pyruvate concentrations.
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