Evidence map›Paper›PMID 20559871›Full record

ArticleTransgenic research2011

Resistance to a DNA and a RNA virus in transgenic plants by using a single chimeric transgene construct.

Ching-Yi Lin, Hsin-Mei Ku, Wen-Shi Tsai, Sylvia K Green, Fuh-Jyh Jan

Abstract read
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In one paragraph

Article in Transgenic research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ching-Yi LinDepartment of Plant Pathology, National Chung Hsing University, Taichung, 402, Taiwan.
Hsin-Mei Ku
Wen-Shi Tsai
Sylvia K Green
Fuh-Jyh Jan

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tomato leaf curl Taiwan virus (ToLCTWV) and Tomato spotted wilt virus (TSWV) are two major tomato viruses that cause serious economic losses. In this study, a partial C2 gene from ToLCTWV and the middle half of the N gene of TSWV were fused as a chimeric transgene to develop multiple virus resistance in transgenic plants. This construct was introduced into Nicotiana benthamiana and tomato by Agrobacterium-mediated transformation. Several transgenic lines showed no symptom post agro-inoculation with ToLCTWV and displayed high resistance to TSWV. The detection of siRNAs indicated that the resistance was via RNA silencing. This study demonstrated that linkage of gene segments from two viruses with distinct genomic organization, one DNA and the other RNA, can confer multiple virus resistance in transgenic plants via gene silencing.

Indexed as

DNA VirusesNicotianaNucleocapsid ProteinsPlant DiseasesPlant LeavesPlants, Genetically ModifiedRecombinant Fusion ProteinsRNA InterferenceRNA, Small InterferingRNA VirusesSolanum lycopersicumTospovirusTransgenesViral ProteinsNucleocapsid ProteinsRecombinant Fusion ProteinsRNA, Small InterferingViral Proteins

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.