ArticleJournal of muscle research and cell motility2010
The role of tropomyosin isoforms and phosphorylation in force generation in thin-filament reconstituted bovine cardiac muscle fibres.
Article in Journal of muscle research and cell motility, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders.International journal of molecular sciences · 2025Review
- Biomechanical evaluation of flash-frozen and cryo-sectioned papillary muscle samples by using sinusoidal analysis: cross-bridge kinetics and the effect of partial CaJournal of muscle research and cell motility · 2024Article
- Assessing Cardiac Contractility From Single Molecules to Whole Hearts.JACC. Basic to translational science · 2024Review
- The effect of gender and obesity in modulating cross-bridge function in cardiac muscle fibers.Journal of muscle research and cell motility · 2022Article
- Acidosis modifies effects of phosphorylated tropomyosin on the actin-myosin interaction in the myocardium.Journal of muscle research and cell motility · 2021Article
- Hypothesis: Single Actomyosin Properties Account for Ensemble Behavior in Active Muscle Shortening and Isometric Contraction.International journal of molecular sciences · 2020Article
- Age, Sex and Overall Health, Measured As Frailty, Modify Myofilament Proteins in Hearts From Naturally Aging Mice.Scientific reports · 2020Article
- Tropomyosin pseudo-phosphorylation results in dilated cardiomyopathy.The Journal of biological chemistry · 2019Article
- Clinically Divergent Mutation Effects on the Structure and Function of the Human Cardiac Tropomyosin Overlap.Biochemistry · 2017Article
- Overexpression of protein kinase STK25 in mice exacerbates ectopic lipid accumulation, mitochondrial dysfunction and insulin resistance in skeletal muscle.Diabetologia · 2017Article
- Impact of tropomyosin isoform composition on fast skeletal muscle thin filament regulation and force development.Journal of muscle research and cell motility · 2015Article
- Significant role of female sex hormones in cardiac myofilament activation in angiotensin II-mediated hypertensive rats.The journal of physiological sciences : JPS · 2014Article
- Phosphorylation of tropomyosin in striated muscle.Journal of muscle research and cell motility · 2013Review
- A study of tropomyosin's role in cardiac function and disease using thin-filament reconstituted myocardium.Journal of muscle research and cell motility · 2013Review
- Tropomyosin Ser-283 pseudo-phosphorylation slows myofibril relaxation.Archives of biochemistry and biophysics · 2013Article
- Deletion of 1-43 amino acids in cardiac myosin essential light chain blunts length dependency of Ca(2+) sensitivity and cross-bridge detachment kinetics.American journal of physiology. Heart and circulatory physiology · 2013Article
- Actin-binding Rho activating protein is expressed in the central nervous system of normal adult rats.Neural regeneration research · 2012Article
- Article
- Structural and functional aspects of the myosin essential light chain in cardiac muscle contraction.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
- Thin filament-reconstituted skinned muscle fibers for the study of muscle physiology.Journal of biomedicine & biotechnology · 2011Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
The thin filament extraction and reconstitution protocol was used to investigate the functional roles of tropomyosin (Tm) isoforms and phosphorylation in bovine myocardium. The thin filament was extracted by gelsolin, reconstituted with G-actin, and further reconstituted with cardiac troponin together with one of three Tm varieties: phosphorylated alphaTm (alphaTm.P), dephosphorylated alphaTm (alphaTm.deP), and dephosphorylated betaTm (betaTm.deP). The effects of Ca, phosphate, MgATP and MgADP concentrations were examined in the reconstituted fibres at pH 7.0 and 25 degrees C. Our data show that Ca(2+) sensitivity (pCa(50): half saturation point) was increased by 0.19 +/- 0.07 units when betaTm.deP was used instead of alphaTm.deP (P < 0.05), and by 0.27 +/- 0.06 units when phosphorylated alphaTm was used (P < 0.005). The cooperativity (Hill factor) decreased (but insignificantly) from 3.2 +/- 0.3 (5) to 2.8 +/- 0.2 (7) with phosphorylation. The cooperativity decreased significantly from 3.2 +/- 0.3 (5) to 2.1 +/- 0.2 (9) with isoform change from alphaTm.deP to betaTm.deP. There was no significant difference in isometric tension or stiffness between alphaTm.P, alphaTm.deP, and betaTm.deP muscle fibres at saturating [Ca(2+)] or after rigor induction. Based on the six-state cross-bridge model, sinusoidal analysis indicated that the equilibrium constants of elementary steps differed up to 1.7x between alphaTm.deP and betaTm.deP, and up to 2.0x between alphaTm.deP and alphaTm.P. The rate constants differed up to 1.5x between alphaTm.deP and betaTm.deP, and up to 2.4x between alphaTm.deP and alphaTm.P. We conclude that tension and stiffness per cross-bridge are not significantly different among the three muscle models.
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Registered trials
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