ArticleArchives of microbiology2010
Environmental and genetic factors that contribute to Escherichia coli K-12 biofilm formation.
Article in Archives of microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- AHL-differential quorum sensing regulation of amino acid metabolism inMicrobiology spectrum · 2024Article
- Metabolomic profiling of bacterial biofilm: trends, challenges, and an emerging antibiofilm target.World journal of microbiology & biotechnology · 2023Review
- Migration Rates on Swim Plates Vary between Escherichia coli Soil Isolates: Differences Are Associated with Variants in Metabolic Genes.Applied and environmental microbiology · 2023Article
- Transposon Insertion in thePathogens (Basel, Switzerland) · 2020Article
- How bacteria recognise and respond to surface contact.FEMS microbiology reviews · 2020Review
- Review
- Characterization of the phosphotransacetylase-acetate kinase pathway for ATP production inJournal of oral microbiology · 2019Article
- Gene Targets in Ocular PathogenicFrontiers in microbiology · 2019Article
- Spectrum-Effect Relationships Between the Bioactive Ingredient ofFrontiers in pharmacology · 2018Article
- Identification and characterization of AckA-dependent protein acetylation in Neisseria gonorrhoeae.PloS one · 2017Article
- Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi.Scientific reports · 2016Article
- Spontaneous mutations in the flhD operon generate motility heterogeneity in Escherichia coli biofilm.BMC microbiology · 2016Article
- Campylobacter jejuni CsrA Regulates Metabolic and Virulence Associated Proteins and Is Necessary for Mouse Colonization.PloS one · 2016Article
- Genetics and Physiology of Acetate Metabolism by the Pta-Ack Pathway of Streptococcus mutans.Applied and environmental microbiology · 2015Article
- BolA is a transcriptional switch that turns off motility and turns on biofilm development.mBio · 2015Article
- OmpR and RcsB abolish temporal and spatial changes in expression of flhD in Escherichia coli biofilm.BMC microbiology · 2013Article
- Article
- (1)H NMR-based metabolite profiling of planktonic and biofilm cells in Acinetobacter baumannii 1656-2.PloS one · 2013Article
- The role of activated acetate intermediates in the control ofWebmedCentral · 2012Article
- Sticky situations: key components that control bacterial surface attachment.Journal of bacteriology · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Biofilms are communities of bacteria whose formation on surfaces requires a large portion of the bacteria's transcriptional network. To identify environmental conditions and transcriptional regulators that contribute to sensing these conditions, we used a high-throughput approach to monitor biofilm biomass produced by an isogenic set of Escherichia coli K-12 strains grown under combinations of environmental conditions. Of the environmental combinations, growth in tryptic soy broth at 37 degrees C supported the most biofilm production. To analyze the complex relationships between the diverse cell-surface organelles, transcriptional regulators, and metabolic enzymes represented by the tested mutant set, we used a novel vector-item pattern-mining algorithm. The algorithm related biofilm amounts to the functional annotations of each mutated protein. The pattern with the best statistical significance was the gene ontology 'pyruvate catabolic process,' which is associated with enzymes of acetate metabolism. Phenotype microarray experiments illustrated that carbon sources that are metabolized to acetyl-coenzyme A, acetyl phosphate, and acetate are particularly supportive of biofilm formation. Scanning electron microscopy revealed structural differences between mutants that lack acetate metabolism enzymes and their parent and confirmed the quantitative differences. We conclude that acetate metabolism functions as a metabolic sensor, transmitting changes in environmental conditions to biofilm biomass and structure.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.