Trial reportJournal of applied physiology (Bethesda, Md. : 1985)2010
Roles of nitric oxide synthase and cyclooxygenase in leg vasodilation and oxygen consumption during prolonged low-intensity exercise in untrained humans.
Trial report in Journal of applied physiology (Bethesda, Md. : 1985), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- Effects of modest hyperoxia and oral vitamin C on exercise hyperaemia and reactive hyperaemia in healthy young men.European journal of applied physiology · 2015Trial
- Increased cGMP improves microvascular exercise training adaptations in diet-induced obesity.American journal of physiology. Endocrinology and metabolism · 2025Article
- Cyclooxygenase inhibition does not blunt thermal hyperemia in skeletal muscle of humans.Journal of applied physiology (Bethesda, Md. : 1985) · 2024Article
- Preserved β-adrenergic-mediated vasodilation in skeletal muscle of young adults with obesity despite shifts in cyclooxygenase and nitric oxide synthase.American journal of physiology. Heart and circulatory physiology · 2022Article
- Effect of histamine-receptor antagonism on leg blood flow during exercise.Journal of applied physiology (Bethesda, Md. : 1985) · 2020Article
- Assessment of resistance vessel function in human skeletal muscle: guidelines for experimental design, Doppler ultrasound, and pharmacology.American journal of physiology. Heart and circulatory physiology · 2020Review
- Rapid-onset vasodilator responses to exercise in humans: Effect of increased baseline blood flow.Experimental physiology · 2020Article
- Skeletal muscle contraction-induced vasodilation in the microcirculation.Journal of exercise rehabilitation · 2017Review
- Comparison of the vasodilatory effects of sodium nitroprusside vs. nitroglycerin.Journal of applied physiology (Bethesda, Md. : 1985) · 2017Article
- Effects of chronic nitric oxide synthase inhibition on V'ONaunyn-Schmiedeberg's archives of pharmacology · 2017Article
- Reduced blood flow to contracting skeletal muscle in ageing humans: is it all an effect of sand through the hourglass?The Journal of physiology · 2016Review
- Skeletal muscle vasodilation during systemic hypoxia in humans.Journal of applied physiology (Bethesda, Md. : 1985) · 2016Review
- Exercise vasodilation is greater in women: contributions of nitric oxide synthase and cyclooxygenase.European journal of applied physiology · 2015Article
- Regulation of increased blood flow (hyperemia) to muscles during exercise: a hierarchy of competing physiological needs.Physiological reviews · 2015Review
- Hybrid diffuse optical techniques for continuous hemodynamic measurement in gastrocnemius during plantar flexion exercise.Journal of biomedical optics · 2015Article
- Thin-beam ultrasound overestimation of blood flow: how wide is your beam?Journal of applied physiology (Bethesda, Md. : 1985) · 2014Article
- Prostaglandins induce vasodilatation of the microvasculature during muscle contraction and induce vasodilatation independent of adenosine.The Journal of physiology · 2014Article
- Human inflammatory and resolving lipid mediator responses to resistance exercise and ibuprofen treatment.American journal of physiology. Regulatory, integrative and comparative physiology · 2013Article
- Effects of prostaglandins and COX-inhibiting drugs on skeletal muscle adaptations to exercise.Journal of applied physiology (Bethesda, Md. : 1985) · 2013Review
- Effect of extraluminal ATP application on vascular tone and blood flow in skeletal muscle: implications for exercise hyperemia.American journal of physiology. Regulatory, integrative and comparative physiology · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The vasodilator signals regulating muscle blood flow during exercise are unclear. We tested the hypothesis that in young adults leg muscle vasodilation during steady-state exercise would be reduced independently by sequential pharmacological inhibition of nitric oxide synthase (NOS) and cyclooxygenase (COX) with NG-nitro-L-arginine methyl ester (L-NAME) and ketorolac, respectively. We tested a second hypothesis that NOS and COX inhibition would increase leg oxygen consumption (VO2) based on the reported inhibition of mitochondrial respiration by nitric oxide. In 13 young adults, we measured heart rate (ECG), blood pressure (femoral venous and arterial catheters), blood gases, and venous oxygen saturation (indwelling femoral venous oximeter) during prolonged (25 min) steady-state dynamic knee extension exercise (60 kick/min, 19 W). Leg blood flow (LBF) was determined by Doppler ultrasound of the femoral artery. Whole body VO2 was measured, and leg VO2 was calculated from blood gases and LBF. Resting intra-arterial infusions of acetylcholine (ACh) and nitroprusside (NTP) tested inhibitor efficacy. Leg vascular conductance (LVC) to ACh was reduced up to 53±4% by L-NAME+ketorolac infusion, and the LVC responses to NTP were unaltered. Exercise increased LVC from 4±1 to 33.1±2 ml.min(-1).mmHg(-1) and tended to decrease after L-NAME infusion (31±2 ml.min(-1).mmHg(-1), P=0.09). With subsequent administration of ketorolac LVC decreased to 29.6±2 ml.min(-1).mmHg(-1) (P=0.02; n=9). While exercise continued, LVC returned to control values (33±2 ml.min(-1).mmHg(-1)) within 3 min, suggesting involvement of additional vasodilator mechanisms. In four additional subjects, LVC tended to decrease with L-NAME infusion alone (P=0.08) but did not demonstrate the transient recovery. Whole body and leg VO2 increased with exercise but were not altered by L-NAME or L-NAME+ketorolac. These data indicate a modest role for NOS- and COX-mediated vasodilation in the leg of exercising humans during prolonged steady-state exercise, which can be restored acutely. Furthermore, NOS and COX do not appear to influence muscle VO2 in untrained healthy young adults.
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