Evidence map›Paper›PMID 20558627›Full record

ArticleAmerican journal of respiratory and critical care medicine2010

Specific T cell frequency and cytokine expression profile do not correlate with protection against tuberculosis after bacillus Calmette-Guérin vaccination of newborns.

Benjamin M N Kagina, Brian Abel, Thomas J Scriba, Elizabeth J Hughes, Alana Keyser, Andreia Soares, Hoyam Gamieldien, Mzwandile Sidibana, Mark Hatherill, Sebastian Gelderbloem and 6 more

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In one paragraph

Article in American journal of respiratory and critical care medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 233 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
233citing papers in PubMed, 4 pooled it
13.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

233 citing papers in PubMed, 4 syntheses or guidelines pooled it, 404 citations in OpenAlex.

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  3. Immunogenicity and Safety of the M72/AS01Frontiers in immunology · 2019
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  17. Immunogenicity of BCG in HIV-exposed and non-exposed infants following routine birth or delayed vaccination.The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease · 2015
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173 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 2 institutions in 2 countries.

Benjamin M N KaginaSouth African Tuberculosis Vaccine Initiative, Institute of Infectious Diseases and Molecular Medicine and School of Child and Adolescent Health, University of Cape Town, Cape Town, South Africa.
Brian Abel
Thomas J Scriba
Elizabeth J Hughes
Alana Keyser
Andreia Soares
Hoyam Gamieldien
Mzwandile Sidibana
Mark Hatherill
Sebastian Gelderbloem
Hassan Mahomed
Anthony Hawkridge
Gregory Hussey
Gilla Kaplan
Willem A Hanekom
other members of the South African Tuberculosis Vaccine Initiative
University of Cape Town · ZAAeras · US

Funding

Protective immunity induced by newborn BCG vaccinationR01AI065653 · NIAID · UNIVERSITY OF CAPE TOWN · PI HANEKOM, WILLEM ALBERT · 2005 to 2009
$2.5M
NIAID NIH HHS R01-AI065653
6 · The paper itself

Abstract

rationaleImmunogenicity of new tuberculosis (TB) vaccines is commonly assessed by measuring the frequency and cytokine expression profile of T cells.

objectivesWe tested whether this outcome correlates with protection against childhood TB disease after newborn vaccination with bacillus Calmette-Guérin (BCG).

methodsWhole blood from 10-week-old infants, routinely vaccinated with BCG at birth, was incubated with BCG for 12 hours, followed by cryopreservation for intracellular cytokine analysis. Infants were followed for 2 years to identify those who developed culture-positive TB-these infants were regarded as not protected against TB. Infants who did not develop TB disease despite exposure to TB in the household, and another group of randomly selected infants who were never evaluated for TB, were also identified-these groups were regarded as protected against TB. Cells from these groups were thawed, and CD4, CD8, and γδ T cell-specific expression of IFN-γ, TNF-α, IL-2, and IL-17 measured by flow cytometry. MEASUREMENTS AND MAIN

resultsA total of 5,662 infants were enrolled; 29 unprotected and two groups of 55 protected infants were identified. There was no difference in frequencies of BCG-specific CD4, CD8, and γδ T cells between the three groups of infants. Although BCG induced complex patterns of intracellular cytokine expression, there were no differences between protected and unprotected infants.

conclusionsThe frequency and cytokine profile of mycobacteria-specific T cells did not correlate with protection against TB. Critical components of immunity against Mycobacterium tuberculosis, such as CD4 T cell IFN-γ production, may not necessarily translate into immune correlates of protection against TB disease.

Indexed as

BCG VaccineCase-Control StudiesCD4 Lymphocyte CountCytokinesHumansImmunophenotypingInfant, NewbornInterferon-gammaProspective StudiesSouth AfricaTuberculosisBCG VaccineCytokinesInterferon-gamma

Identifiers

PMID20558627
PMCPMC2970848
OpenAlexW2150707766

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.