ArticleThe Journal of nutrition2010
Candidate dietary phytochemicals modulate expression of phase II enzymes GSTP1 and NQO1 in human lung cells.
Article in The Journal of nutrition, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 56 citations in OpenAlex.
- Exploring the Relationship Between Dietary Phytochemical Index and Chemotherapy-Related Symptoms: Insights From a Cross-Sectional Study.Food science & nutrition · 2024Article
- Design and Evaluation of Liposomal Sulforaphane-Loaded Polyvinyl Alcohol/Polyethylene Glycol (PVA/PEG) Hydrogels as a Novel Drug Delivery System for Wound Healing.Gels (Basel, Switzerland) · 2023Article
- A review on anti-nutritional factors: unraveling the natural gateways to human health.Frontiers in nutrition · 2023Review
- Direct Inhibition of GSDMD by PEITC Reduces Hepatocyte Pyroptosis and Alleviates Acute Liver Injury in Mice.Frontiers in immunology · 2022Article
- Discovery of a dual inhibitor of NQO1 and GSTP1 for treating glioblastoma.Journal of hematology & oncology · 2020Article
- The Role of Isothiocyanates as Cancer Chemo-Preventive, Chemo-Therapeutic and Anti-Melanoma Agents.Antioxidants (Basel, Switzerland) · 2019Review
- Decaffeinated Green Tea Extract Does Not Elicit Hepatotoxic Effects and Modulates the Gut Microbiome in Lean B6C3F₁ Mice.Nutrients · 2019Article
- Inhibition of glutathione S-transferase-pi triggers c-jun N-terminal kinase-dependent neuronal death in Zn-induced Parkinsonism.Molecular and cellular biochemistry · 2019Article
- Preventive effects of combinative natural foods produced by elite crop varieties rich in anticancer effects onFood science & nutrition · 2019Article
- Anti-Carcinogenic Glucosinolates in Cruciferous Vegetables and Their Antagonistic Effects on Prevention of Cancers.Molecules (Basel, Switzerland) · 2018Review
- The Role of Glucosinolate Hydrolysis Products from Brassica Vegetable Consumption in Inducing Antioxidant Activity and Reducing Cancer Incidence.Diseases (Basel, Switzerland) · 2016Review
- Respiratory protease/antiprotease balance determines susceptibility to viral infection and can be modified by nutritional antioxidants.American journal of physiology. Lung cellular and molecular physiology · 2015Review
- CDK14 expression is down-regulated by cigarette smoke in vivo and in vitro.Toxicology letters · 2015Article
- Effects of benzyl isothiocyanate and its N-acetylcysteine conjugate on induction of detoxification enzymes in hepa1c1c7 mouse hepatoma cells.Preventive nutrition and food science · 2014Article
- Reference genes for real-time PCR quantification of messenger RNAs and microRNAs in mouse model of obesity.PloS one · 2014Article
- Major differences among chemopreventive organoselenocompounds in the sustained elevation of cytoprotective genes.Journal of biochemical and molecular toxicology · 2012Article
- EGCG protects endothelial cells against PCB 126-induced inflammation through inhibition of AhR and induction of Nrf2-regulated genes.Toxicology and applied pharmacology · 2012Article
- High-throughput library screening identifies two novel NQO1 inducers in human lung cells.American journal of respiratory cell and molecular biology · 2012Article
- Notch activation is dispensable for D, L-sulforaphane-mediated inhibition of human prostate cancer cell migration.PloS one · 2012Article
- Encapsulated nanoepigallocatechin-3-gallate and elemental selenium nanoparticles as paradigms for nanochemoprevention.International journal of nanomedicine · 2012Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Many phytochemicals possess cancer-preventive properties, some putatively through phase II metabolism-mediated mutagen/oxidant quenching. We applied human lung cells in vitro to investigate the effects of several candidate phytopreventive agents, including green tea extracts (GTE), broccoli sprout extracts (BSE), epigallocatechin gallate (EGCG), sulforaphane (SFN), phenethyl isothiocyanate (PEITC), and benzyl isothiocyanate (BITC), on inducing phase II enzymes glutathione S-transferase P1 (GSTP1) and NAD(P)H:quinone oxidoreductase 1 (NQO1) at mRNA and protein levels. Primary normal human bronchial epithelial cells (NHBE), immortalized human bronchial epithelial cells (HBEC), and lung adenocarcinoma cells (A549) were exposed to diet-achievable levels of GTE and BSE (0.5, 1.0, 2.0 mg/L), or individual index components EGCG, SFN, PEITC, BITC (0.5, 1.0, 2.0 micromol/L) for 24 h, 48 h, and 6 d, respectively. mRNA assays employed RNA-specific quantitative RT-PCR and protein assays employed Western blotting. We found that in NHBE cells, while GSTP1 mRNA levels were slightly but significantly increased after exposure to GTE or BSE, NQO1 mRNA increased to 2- to 4-fold that of control when exposed to GTE, BSE, or SFN. Effects on NQO1 mRNA expression in HBEC cells were similar. NQO1 protein expression increased up to 11.8-fold in SFN-treated NHBE cells. Both GSTP1 and NQO1 protein expression in A549 cells were constitutively high but not induced under any condition. Our results suggest that NQO1 is more responsive to the studied chemopreventive agents than GSTP1 in human lung cells and there is discordance between single agent and complex mixture effects. We conclude that modulation of lung cell phase II metabolism by chemopreventive agents requires cell- and agent-specific discovery and testing.
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