ArticleNucleic acids research2010
Dual roles for the Mss116 cofactor during splicing of the ai5γ group II intron.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Novel Quinazoline Derivatives Inhibit Splicing of Fungal Group II Introns.ACS chemical biology · 2025Article
- Article
- Discovery of highly reactive self-splicing group II introns within the mitochondrial genomes of human pathogenic fungi.Nucleic acids research · 2021Article
- Article
- DEAD-box protein CYT-19 is activated by exposed helices in a group I intron RNA.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- RNA helicase proteins as chaperones and remodelers.Annual review of biochemistry · 2014Review
- The DEAD-box protein Dbp2 functions with the RNA-binding protein Yra1 to promote mRNP assembly.Journal of molecular biology · 2013Article
- Predicted group II intron lineages E and F comprise catalytically active ribozymes.RNA (New York, N.Y.) · 2013Article
- Mss116p: a DEAD-box protein facilitates RNA folding.RNA biology · 2013Review
- Toward a molecular understanding of RNA remodeling by DEAD-box proteins.RNA biology · 2013Review
- The brace for a growing scaffold: Mss116 protein promotes RNA folding by stabilizing an early assembly intermediate.Journal of molecular biology · 2012Article
- RNA catalysis as a probe for chaperone activity of DEAD-box helicases.Methods in enzymology · 2012Article
- The Azoarcus group I intron ribozyme misfolds and is accelerated for refolding by ATP-dependent RNA chaperone proteins.The Journal of biological chemistry · 2011Article
- RNA helicases and remodeling proteins.Current opinion in chemical biology · 2011Review
- ATP-dependent roles of the DEAD-box protein Mss116p in group II intron splicing in vitro and in vivo.Journal of molecular biology · 2011Article
- Mechanism of Mss116 ATPase reveals functional diversity of DEAD-Box proteins.Journal of molecular biology · 2011Article
- Emerging structural themes in large RNA molecules.Current opinion in structural biology · 2011Review
- Article
- DEAD-box proteins as RNA helicases and chaperones.Wiley interdisciplinary reviews. RNAReview
- Article
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3 authors.
Funding
Abstract
The autocatalytic group II intron ai5γ from Saccharomyces cerevisiae self-splices under high-salt conditions in vitro, but requires the assistance of the DEAD-box protein Mss116 in vivo and under near-physiological conditions in vitro. Here, we show that Mss116 influences the folding mechanism in several ways. By comparing intron precursor RNAs with long (∼300 nt) and short (∼20 nt) exons, we observe that long exon sequences are a major obstacle for self-splicing in vitro. Kinetic analysis indicates that Mss116 not only mitigates the inhibitory effects of long exons, but also assists folding of the intron core. Moreover, a mutation in conserved Motif III that impairs unwinding activity (SAT → AAA) only affects the construct with long exons, suggesting helicase unwinding during exon unfolding, but not in intron folding. Strong parallels between Mss116 and the related protein Cyt-19 from Neurospora crassa suggest that these proteins form a subclass of DEAD-box proteins that possess a versatile repertoire of diverse activities for resolving the folding problems of large RNAs.
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