ArticleJournal of clinical microbiology2010
Detection and characterization of human Ureaplasma species and serovars by real-time PCR.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed.
- Toll-like receptor variants and cervical Atopobium vaginae infection in women with pelvic inflammatory disease.American journal of reproductive immunology (New York, N.Y. : 1989) · 2018Trial
- Identification of novel microbes associated with pelvic inflammatory disease and infertility.Sexually transmitted infections · 2016Trial
- Pharmacokinetics, microbial response, and pulmonary outcomes of multidose intravenous azithromycin in preterm infants at risk for Ureaplasma respiratory colonization.Antimicrobial agents and chemotherapy · 2015Trial
- Azithromycin to prevent bronchopulmonary dysplasia in ureaplasma-infected preterm infants: pharmacokinetics, safety, microbial response, and clinical outcomes with a 20-milligram-per-kilogram single intravenous dose.Antimicrobial agents and chemotherapy · 2013Trial
- Hyperammonaemic encephalopathy and subsequent arthritis due to Ureaplasma urealyticum in a haematopoietic cell transplant recipient: a case report and literature review.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- InvasiveASM case reports · 2026Article
- Head trauma complicated with multi-site infections of Ureaplasma urealyticum and Mycoplasma hominis: a case report.BMC infectious diseases · 2025Article
- Antimicrobial susceptibility and genetic mechanisms of resistance ofAntimicrobial agents and chemotherapy · 2025Article
- Prostatic Abscess due toCase reports in infectious diseases · 2025Article
- Donor-derived Mycoplasma and Ureaplasma infections in lung transplant recipients: A prospective study of donor and recipient respiratory tract screening and recipient outcomes.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2024Article
- Membrane inflammasome activation by choriodecidual Ureaplasma parvum infection without intra-amniotic infection in a Non-Human Primate model†.Biology of reproduction · 2024Article
- Risk factors, management, and clinical outcomes of invasive Mycoplasma and Ureaplasma infections after lung transplantation.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2024Article
- Sexually Transmitted Infections in Male Patients with Urethritis.Pathogens (Basel, Switzerland) · 2023Article
- MYCO WELL D-ONE detection of Ureaplasma spp. and Mycoplasma hominis in sexual health patients in Wales.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- Aetiology and prevalence of mixed-infections and mono-infections in non-gonococcal urethritis in men: a case-control study.Sexually transmitted infections · 2020Article
- Presence and Concentrations of Select Bacterial Vaginosis-Associated Bacteria Are Associated With Increased Risk of Pelvic Inflammatory Disease.Sexually transmitted diseases · 2020Article
- GrpE Immunization Protects AgainstFrontiers in immunology · 2020Article
- Detection of Ureaplasma spp. serovars in genital tract of infertile males.Journal of clinical laboratory analysis · 2019Article
- PerinatalFrontiers in cellular and infection microbiology · 2019Article
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
We designed primers and probes for the detection and discrimination of Ureaplasma parvum and U. urealyticum and their 14 serovars by real-time PCR. The analytical sensitivity and specificity of the multiplex species-specific PCR were determined by testing corresponding American Type Culture Collection (ATCC) type strains, 47 other microbial species, and human genomic DNA. The limits of the multiplex PCR were 2.8 x 10(-2) CFU/microl PCR mixture for detecting U. parvum and 4.1 x 10(-2) CFU/microl PCR mixture for detecting U. urealyticum. Clinical specificity and sensitivity were proven by comparison with culture and traditional PCR. For the detection of any Ureaplasma species, the clinical sensitivity and specificity of real-time PCR were 96.9% and 79.0%, respectively, using culture as a reference. Multiplex real-time PCR was also more sensitive than traditional PCR in discriminating the two Ureaplasma species in culture-positive subcultures. Each of the 14 monoplex serovar-specific PCR assays was specific for the corresponding ATCC type strain serovar. This new species identification PCR is specific and sensitive in the detection of Ureaplasma species in clinical specimens, and the serovar-specific PCR assays are the first set of complete genotypic assays to differentiate all 14 known Ureaplasma serovars. These assays provide quick and reliable means for investigating the epidemiology and pathogenicity of ureaplasmas at the serovar level.
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Registered trials
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