ArticleJournal of clinical microbiology2010
Genome-wide single nucleotide polymorphism typing method for identification of Bacillus anthracis species and strains among B. cereus group species.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Identification ofPathogens (Basel, Switzerland) · 2025Article
- Microbial sequence typing in the genomic era.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2018Review
- Development of a High Resolution Virulence Allelic Profiling (HReVAP) Approach Based on the Accessory Genome of Escherichia coli to Characterize Shiga-Toxin Producing E. coli (STEC).Frontiers in microbiology · 2016Article
- Whole Genome-Sequencing and Phylogenetic Analysis of a Historical Collection of Bacillus anthracis Strains from Danish Cattle.PloS one · 2015Article
- Source tracking of an anthrax outbreak in northeastern China using complete genome analysis and MLVA genotyping.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Population analysis of Streptococcus suis isolates from slaughtered swine by use of minimum core genome sequence typing.Journal of clinical microbiology · 2014Article
- Bifidobacterium animalis subsp. lactis ATCC 27673 is a genomically unique strain within its conserved subspecies.Applied and environmental microbiology · 2013Article
- Bacillus "next generation" diagnostics: moving from detection toward subtyping and risk-related strain profiling.Frontiers in microbiology · 2013Article
- Article
- Temporal differences of onset between primary skin lesions and regional lymph node lesions for tularemia in Japan: a clinicopathologic and immunohistochemical study of 19 skin cases and 54 lymph node cases.Virchows Archiv : an international journal of pathology · 2012Article
- Insights from genomic comparisons of genetically monomorphic bacterial pathogens.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Review
- Development of a temperature-switch PCR-based SNP typing method for Mycobacterium ulcerans.PLoS neglected tropical diseases · 2012Article
- MLVA and SNP analysis identified a unique genetic cluster in Bulgarian Bacillus anthracis strains.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Article
- Fourteen-genome comparison identifies DNA markers for severe-disease-associated strains of Clostridium difficile.Journal of clinical microbiology · 2011Article
- Genome sequencing and analysis of Yersina pestis KIM D27, an avirulent strain exempt from select agent regulation.PloS one · 2011Article
- You cannot B. cereus.Nature reviews. Microbiology · 2011Article
- SNP/RD typing of Mycobacterium tuberculosis Beijing strains reveals local and worldwide disseminated clonal complexes.PloS one · 2011Article
- Genomic tracing of epidemics and disease outbreaks.Microbial biotechnology · 2010Article
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As an issue of biosecurity, species-specific genetic markers have been well characterized. However, Bacillus anthracis strain-specific information is currently not sufficient for traceability to identify the origin of the strain. By using genome-wide screening using short read mapping, we identified strain-specific single nucleotide polymorphisms (SNPs) among B. anthracis strains including Japanese isolates, and we further developed a simplified 80-tag SNP typing method for the primary investigation of traceability. These 80-tag SNPs were selected from 2,965 SNPs on the chromosome and the pXO1 and pXO2 plasmids from a total of 19 B. anthracis strains, including the available genome sequences of 17 strains in the GenBank database and 2 Japanese isolates that were sequenced in this study. Phylogenetic analysis based on 80-tag SNP typing showed a higher resolution power to discriminate 12 Japanese isolates rather than the 25 loci identified by multiple-locus variable-number tandem-repeat analysis (MLVA). In addition, the 80-tag PCR testing enabled the discrimination of B. anthracis from other B. cereus group species, helping to identify whether a suspected sample originates from the intentional release of a bioterrorism agent or environmental contamination with a virulent agent. In conclusion, 80-tag SNP typing can be a rapid and sufficient test for the primary investigation of strain origin. Subsequent whole-genome sequencing will reveal apparent strain-specific genetic markers for traceability of strains following an anthrax outbreak.
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