ArticleJournal of clinical microbiology2010
Epidemiological and phylogenetic analysis of Spanish human Brucella melitensis strains by multiple-locus variable-number tandem-repeat typing, hypervariable octameric oligonucleotide fingerprinting, and rpoB typing.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Core Genome Multilocus Sequence Typing Scheme for Improved Characterization and Epidemiological Surveillance of Pathogenic Brucella.Journal of clinical microbiology · 2022Article
- Genetic Characterization ofFrontiers in microbiology · 2021Article
- Phenotypic and genotypic characteristics of Brucella isolates from the Republic of Kazakhstan.Tropical animal health and production · 2019Article
- Using molecular tools to identify the geographical origin of a case of human brucellosis.Epidemiology and infection · 2015Article
- Full genome SNP-based phylogenetic analysis reveals the origin and global spread of Brucella melitensis.BMC genomics · 2015Article
- Genotyping of human Brucella melitensis biovar 3 isolated from Shanxi Province in China by MLVA16 and HOOF.PloS one · 2015Article
- Isolation and molecular characterization of Brucella isolates in cattle milk in Uganda.BioMed research international · 2015Article
- Human brucellosis in Maghreb: existence of a lineage related to socio-historical connections with Europe.PloS one · 2014Article
- Article
- Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis.BioMed research international · 2014Article
- Within-host evolution of Brucella canis during a canine brucellosis outbreak in a kennel.BMC veterinary research · 2013Article
- Biotyping and genotyping (MLVA16) of Brucella abortus isolated from cattle in Brazil, 1977 to 2008.PloS one · 2013Article
- Article
- Molecular typing of Brucella melitensis endemic strains and differentiation from the vaccine strain Rev-1.Veterinary research communications · 2012Article
- MLVA16 typing of Portuguese human and animal Brucella melitensis and Brucella abortus isolates.PloS one · 2012Article
- MLVA genotyping of Chinese human Brucella melitensis biovar 1, 2 and 3 isolates.BMC microbiology · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The severe morbidity of human brucellosis is one of the main reasons for using molecular typing in the epidemiological surveillance of this worldwide zoonosis. Multiple-locus variable-number repeat analysis (MLVA-16), hypervariable octameric oligonucleotide fingerprinting (HOOF-print), and the differences in the single nucleotide polymorphisms (SNPs) (codons 1249 and 1309) of the DNA-dependent RNA polymerase beta subunit (rpoB) were used to type a human Brucella melitensis population (108 strains) collected from throughout Spain over 13 years. Eighty-six MLVA types (discriminatory index, 0.99) were detected, with a wide-ranging genetic similarity coefficient (37.2 to 93.7%). The population clustered into the following groups: American, with genotypes 47 (1 strain), 48 (13 strains), 53 (12 strains), 55 (2 strains), 80 (1 strain), and a new genotype (2 strains), Western Mediterranean, with genotype 51 (9 strains), and Eastern Mediterranean, with genotypes 42 (60 strains), 43 (4 strains), and 63 (4 strains). Two profession-related and two foodborne acquisitions were confirmed. Distributed throughout Spain, Eastern Mediterranean genotype 42 was the most common (55%). The low MLVA-16 allelic polymorphism (genetic similarity range, 75 to 94%) of the genotype 42 strains suggests that they recently evolved from a common ancestor. rpoB typing grouped the strains as rpoB type 1 (1249-ATG/1309-CTG; 28.7%), rpoB type 2 (1249-ATG/1309-CTA; 62.9%), and rpoB type 3 (1249-ATA/1309-CTG; 8.3%). According to the MLVA-16 results, the population clustered by rpoB type. Given the correlation between B. melitensis MLVA groups and rpoB types (American and rpoB type 1, Eastern Mediterranean and rpoB type 2, and Western Mediterranean and rpoB type 3), the rpoB type could be used as an initial marker for the epidemiological surveillance of brucellosis.
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