ArticleJournal of clinical microbiology2010
Comparison of an automated repetitive-sequence-based PCR microbial typing system with pulsed-field gel electrophoresis for molecular typing of vancomycin-resistant Enterococcus faecium.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Comparative analysis of virulence genes, biofilm production, and antibiotic resistance in Escherichia coli from dogs and humans using rep-PCR.BMC veterinary research · 2025Article
- Predictors for vancomycin resistantAntimicrobial resistance and infection control · 2019Article
- Molecular Characterization Of Vancomycin-ResistantInfection and drug resistance · 2019Article
- First molecular characterization of related cases of healthcare-associated infections involving multidrug-resistantInfection and drug resistance · 2018Article
- Outbreak of vancomycin-resistantJournal of infection prevention · 2017Article
- Molecular characterization of vancomycin-resistant Enterococcus faecium isolates from Bermuda.PloS one · 2017Article
- Genetic characteristics and molecular epidemiology of vancomycin-resistant Enterococci isolates from Caribbean countries.PloS one · 2017Article
- Molecular Analysis of Vancomycin-Resistant Enterococci Isolated from Regional Hospitals in Trinidad and Tobago.Advances in medicine · 2016Article
- Comparison of a semiautomated commercial repetitive-sequence-based PCR method with spoligotyping, 24-locus mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing, and restriction fragment length polymorphism-based analysis of IS6110 for Mycobacterium tuberculosis typing.Journal of clinical microbiology · 2014Article
- Investigation of vancomycin resistant Enterococcus faecium outbreak in neonatal intensive care unit.International journal of clinical and experimental medicine · 2014Article
- Beyond the bundle--journey of a tertiary care medical intensive care unit to zero central line-associated bloodstream infections.Critical care (London, England) · 2013Observational
- A Web-based multidrug-resistant organisms surveillance and outbreak detection system with rule-based classification and clustering.Journal of medical Internet research · 2012Article
- Raman spectroscopic analysis of the clonal and horizontal spread of CTX-M-15-producing Klebsiella pneumoniae in a neonatal intensive care unit.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Molecular methods for pathogen and microbial community detection and characterization: current and potential application in diagnostic microbiology.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2012Review
- Incidence of and risk factors for infection or colonization of vancomycin-resistant enterococci in patients in the intensive care unit.PloS one · 2012Article
- Controlled performance evaluation of the DiversiLab repetitive-sequence-based genotyping system for typing multidrug-resistant health care-associated bacterial pathogens.Journal of clinical microbiology · 2011Article
- High-resolution melting genotyping of Enterococcus faecium based on multilocus sequence typing derived single nucleotide polymorphisms.PloS one · 2011Article
- Effectiveness and limitations of hand hygiene promotion on decreasing healthcare-associated infections.PloS one · 2011Article
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4 authors.
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Abstract
Vancomycin-resistant Enterococcus faecium (VRE) has become an important health care-associated pathogen because of its rapid spread, limited therapeutic options, and possible transfer of vancomycin resistance to more-virulent pathogens. In this study, we compared the ability to detect clonal relationships among VRE isolates by an automated repetitive-sequence-based PCR (Rep-PCR) system (DiversiLab system) to pulsed-field gel electrophoresis (PFGE), the reference method for molecular typing of VRE. Two sets of VRE isolates evaluated in this study were collected by active microbial surveillance at a large teaching hospital in Taiwan during 2008. The first set included 90 isolates randomly selected from the surveillance cohort. The first set consisted of 34 pulsotypes and 10 Rep-PCR types. There was good correlation between the two methods (P < 0.001). The second set included 68 VRE isolates collected from eight clusters of colonization. A dominant clone was detected in five out of eight clusters by both methods. Two clusters were characterized by Rep-PCR as being caused by a dominant clone, whereas PFGE showed polyclonal origins. One cluster was shown to be polyclonal by both methods. A single Rep-PCR clone type was detected among 12 of 14 vancomycin-intermediate enterococci, whereas PFGE detected six pulsotypes. In conclusion, the Rep-PCR method correlated well with PFGE typing but was less discriminative than PFGE in defining clonal relationships. The ease of use and more rapid turnaround time of Rep-PCR compared to PFGE offers a rapid screening method to detect outbreaks of VRE and more rapidly implement control measures. PFGE remains the preferred method to confirm clonal spread.
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