ArticleThe Journal of biological chemistry2010
Involvement of Grb2 adaptor protein in nucleophosmin-anaplastic lymphoma kinase (NPM-ALK)-mediated signaling and anaplastic large cell lymphoma growth.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
- Article
- ALK in cancer: from function to therapeutic targeting.Nature reviews. Cancer · 2025Review
- The Configuration of GRB2 in Protein Interaction and Signal Transduction.Biomolecules · 2024Review
- Defining Pathological Activities of ALK in Neuroblastoma, a Neural Crest-Derived Cancer.International journal of molecular sciences · 2021Review
- Aberrant role of ALK in tau proteinopathy through autophagosomal dysregulation.Molecular psychiatry · 2021Article
- Article
- The 11-Kilodalton Nonstructural Protein of Human Parvovirus B19 Facilitates Viral DNA Replication by Interacting with Grb2 through Its Proline-Rich Motifs.Journal of virology · 2019Article
- Coupling an EML4-ALK-centric interactome with RNA interference identifies sensitizers to ALK inhibitors.Science signaling · 2016Article
- Grb2 monomer-dimer equilibrium determines normal versus oncogenic function.Nature communications · 2015Article
- Pleiotrophin mediates hematopoietic regeneration via activation of RAS.The Journal of clinical investigation · 2014Article
- ALK and crizotinib: after the honeymoon…what else? Resistance mechanisms and new therapies to overcome it.Translational lung cancer research · 2014Review
- Molecular and functional characterizations of the association and interactions between nucleophosmin-anaplastic lymphoma kinase and type I insulin-like growth factor receptor.Neoplasia (New York, N.Y.) · 2013Article
- NPM-ALK up-regulates iNOS expression through a STAT3/microRNA-26a-dependent mechanism.The Journal of pathology · 2013Article
- ALK Signaling and Target Therapy in Anaplastic Large Cell Lymphoma.Frontiers in oncology · 2012Article
- NPM-ALK: The Prototypic Member of a Family of Oncogenic Fusion Tyrosine Kinases.Journal of signal transduction · 2012Article
- The potential for crizotinib in non-small cell lung cancer: a perspective review.Therapeutic advances in medical oncology · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Most anaplastic large cell lymphomas (ALCL) express oncogenic fusion proteins derived from chromosomal translocations or inversions of the anaplastic lymphoma kinase (ALK) gene. Frequently ALCL carry the t(2;5) translocation, which fuses the ALK gene to the nucleophosmin (NPM1) gene. The transforming activity mediated by NPM-ALK fusion induces different pathways that control proliferation and survival of lymphoma cells. Grb2 is an adaptor protein thought to play an important role in ALK-mediated transformation, but its interaction with NPM-ALK, as well as its function in regulating ALCL signaling pathways and cell growth, has never been elucidated. Here we show that active NPM-ALK, but not a kinase-dead mutant, bound and induced Grb2 phosphorylation in tyrosine 160. An intact SH3 domain at the C terminus of Grb2 was required for Tyr(160) phosphorylation. Furthermore, Grb2 did not bind to a single region but rather to different regions of NPM-ALK, mainly Tyr(152-156), Tyr(567), and a proline-rich region, Pro(415-417). Finally, shRNA knockdown experiments showed that Grb2 regulates primarily the NPM-ALK-mediated phosphorylation of SHP2 and plays a key role in ALCL cell growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.