ArticleBiochimica et biophysica acta2010
Identification of Yju3p as functional orthologue of mammalian monoglyceride lipase in the yeast Saccharomycescerevisiae.
Article in Biochimica et biophysica acta, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 66 citations in OpenAlex.
- Prioritization of chemical scaffolds using the TDR Targets database: An integrative workflow for Trypanosoma cruzi drug discovery.PLoS neglected tropical diseases · 2026Article
- Engineering yeast for tailored fatty acid profiles.Applied microbiology and biotechnology · 2025Review
- Discovery of a non-canonical prototype long-chain monoacylglycerol lipase through a structure-based endogenous reaction intermediate complex.Nature communications · 2023Article
- Harnessing Clickable Acylated Glycerol Probes as Chemical Tools for Tracking Glycerolipid Metabolism.Chemistry (Weinheim an der Bergstrasse, Germany) · 2023Article
- Metabolic, Organoleptic and Transcriptomic Impact ofInternational journal of molecular sciences · 2021Article
- Article
- Tapetal Expression ofFrontiers in plant science · 2019Article
- Lipid droplet-mediated lipid and protein homeostasis in budding yeast.FEBS letters · 2018Review
- Monoacylglycerol Lipases Act as Evolutionarily Conserved Regulators of Non-oxidative Ethanol Metabolism.The Journal of biological chemistry · 2016Article
- Lipid droplet dynamics in budding yeast.Cellular and molecular life sciences : CMLS · 2015Review
- Purification, crystallization and preliminary X-ray diffraction analysis of a soluble variant of the monoglyceride lipase Yju3p from the yeast Saccharomyces cerevisiae.Acta crystallographica. Section F, Structural biology communications · 2015Article
- A peptide derived from G0/G1 switch gene 2 acts as noncompetitive inhibitor of adipose triglyceride lipase.The Journal of biological chemistry · 2014Article
- High confidence proteomic analysis of yeast LDs identifies additional droplet proteins and reveals connections to dolichol synthesis and sterol acetylation.Journal of lipid research · 2014Article
- Screening for hydrolytic enzymes reveals Ayr1p as a novel triacylglycerol lipase in Saccharomyces cerevisiae.The Journal of biological chemistry · 2013Article
- Conformational plasticity and ligand binding of bacterial monoacylglycerol lipase.The Journal of biological chemistry · 2013Article
- Revising the Representation of Fatty Acid, Glycerolipid, and Glycerophospholipid Metabolism in the Consensus Model of Yeast Metabolism.Industrial biotechnology (New Rochelle, N.Y.) · 2013Article
- The evolutionarily conserved protein CG9186 is associated with lipid droplets, required for their positioning and for fat storage.Journal of cell science · 2013Article
- Lipid droplets and peroxisomes: key players in cellular lipid homeostasis or a matter of fat--store 'em up or burn 'em down.Genetics · 2013Review
- A bifunctional enzyme that has both monoacylglycerol acyltransferase and acyl hydrolase activities.Plant physiology · 2012Article
- High level expression and characterization of a thermostable lysophospholipase from Thermococcus kodakarensis KOD1.Extremophiles : life under extreme conditions · 2012Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Monoacylglycerols (MAGs) are short-lived intermediates of glycerolipid metabolism. Specific molecular species, such as 2-arachidonoylglycerol, which is a potent activator of cannabinoid receptors, may also function as lipid signaling molecules. In mammals, enzymes hydrolyzing MAG to glycerol and fatty acids, resembling the final step in lipolysis, or esterifying MAG to diacylglycerol, are well known; however, despite the high level of conservation of lipolysis, the corresponding activities in yeast have not been characterized yet. Here we provide evidence that the protein Yju3p functions as a potent MAG hydrolase in yeast. Cellular MAG hydrolase activity was decreased by more than 90% in extracts of Yju3p-deficient cells, indicating that Yju3p accounts for the vast majority of this activity in yeast. Loss of this activity was restored by heterologous expression of murine monoglyceride lipase (MGL). Since yju3Delta mutants accumulated MAG in vivo only at very low concentrations, we considered the possibility that MAGs are re-esterified into DAG by acyltransferases. Indeed, cellular MAG levels were further increased in mutant cells lacking Yju3p and Dga1p or Lro1p acyltransferase activities. In conclusion, our studies suggest that catabolic and anabolic reactions affect cellular MAG levels. Yju3p is the functional orthologue of mammalian MGL and is required for efficient degradation of MAG in yeast.
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