ReviewMethods (San Diego, Calif.)2010
Use of the spliceosomal protein U1A to facilitate crystallization and structure determination of complex RNAs.
Review in Methods (San Diego, Calif.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 54 citations in OpenAlex.
- Scaffolds with optimized quaternary symmetry for de novo cryoEM structure determination of small RNAs.Nature methods · 2026Article
- A general strategy for engineering GU base pairs to facilitate RNA crystallization.Nucleic acids research · 2025Article
- RNA target highlights in CASP15: Evaluation of predicted models by structure providers.Proteins · 2023Article
- General Strategies for RNA X-ray Crystallography.Molecules (Basel, Switzerland) · 2023Review
- Sub-3-Å cryo-EM structure of RNA enabled by engineered homomeric self-assembly.Nature methods · 2022Article
- Obtaining Crystals of Nucleic Acids in Complex with the Protein U1A Using the Soaking Method.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Structures of artificially designed discrete RNA nanoarchitectures at near-atomic resolution.Science advances · 2021Article
- Engineering Crystal Packing in RNA Structures I: Past and Future Strategies for Engineering RNA Packing in Crystals.Crystals · 2021Article
- Affinity and Structural Analysis of the U1A RNA Recognition Motif with Engineered Methionines to Improve Experimental Phasing.Crystals · 2021Article
- Improving RNA Crystal Diffraction Quality by Postcrystallization Treatment.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Development of imaging scaffolds for cryo-electron microscopy.Current opinion in structural biology · 2020Review
- Exploring the molecular basis of RNA recognition by the dimeric RNA-binding protein via molecular simulation methods.RNA biology · 2016Article
- Inforna 2.0: A Platform for the Sequence-Based Design of Small Molecules Targeting Structured RNAs.ACS chemical biology · 2016Article
- Use of the U1A Protein to Facilitate Crystallization and Structure Determination of Large RNAs.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Post-crystallization Improvement of RNA Crystal Diffraction Quality.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Crystal structure of a c-di-AMP riboswitch reveals an internally pseudo-dimeric RNA.The EMBO journal · 2014Article
- Dramatic improvement of crystals of large RNAs by cation replacement and dehydration.Structure (London, England : 1993) · 2014Article
- New molecular engineering approaches for crystallographic studies of large RNAs.Current opinion in structural biology · 2014Review
- Crystallographic analysis of TPP riboswitch binding by small-molecule ligands discovered through fragment-based drug discovery approaches.Methods in enzymology · 2014Article
- Engineering RNA-binding proteins for biology.The FEBS journal · 2013Review
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
The structure determination of complex RNA molecules such as ribozymes, riboswitches and aptamers by X-ray crystallography hinges on the preparation of well-ordered crystals. Success usually results from molecular engineering to facilitate crystallization. An approach that has resulted in 10 new RNA structures in the past decade is the use of the U1A crystallization module. In this approach, the cognate site for the U1A spliceosomal protein is introduced into a functionally dispensable location in the RNA of interest, and the RNA is cocrystallized with the basic RNA-binding protein. In addition to facilitating crystallization, the presence of U1A can be useful for de novo phase determination. In this paper, some general considerations for the use of this approach to RNA crystallization are presented, and specifics of the application of the U1A module to the crystallization of the hairpin ribozyme and the tetracycline aptamer are reviewed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.