ReviewMethods (San Diego, Calif.)2010
Rapid global structure determination of large RNA and RNA complexes using NMR and small-angle X-ray scattering.
Review in Methods (San Diego, Calif.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Advances in solid-state NMR methods for studying RNA structures and dynamics.Magnetic resonance letters · 2025Review
- Biological Magnetic Resonance Data Bank.Nucleic acids research · 2023Article
- NMR Studies of Retroviral Genome Packaging.Viruses · 2020Review
- A Mn-sensing riboswitch activates expression of a Mn2+/Ca2+ ATPase transporter in Streptococcus.Nucleic acids research · 2019Article
- Long-Range RNA Structural Information via a Paramagnetically Tagged Reporter Protein.Journal of the American Chemical Society · 2019Article
- Incorporation of isotopic, fluorescent, and heavy-atom-modified nucleotides into RNAs by position-selective labeling of RNA.Nature protocols · 2018Article
- Structure of the 30 kDa HIV-1 RNA Dimerization Signal by a Hybrid Cryo-EM, NMR, and Molecular Dynamics Approach.Structure (London, England : 1993) · 2018Article
- Structure modeling of RNA using sparse NMR constraints.Nucleic acids research · 2017Article
- Analysis of RNA structure using small-angle X-ray scattering.Methods (San Diego, Calif.) · 2017Article
- Applications of PLOR in labeling large RNAs at specific sites.Methods (San Diego, Calif.) · 2016Article
- Hybrid Approaches to Structural Characterization of Conformational Ensembles of Complex Macromolecular Systems Combining NMR Residual Dipolar Couplings and Solution X-ray Scattering.Chemical reviews · 2016Review
- Structural Analysis of Multi-Helical RNAs by NMR-SAXS/WAXS: Application to the U4/U6 di-snRNA.Journal of molecular biology · 2016Article
- Small-angle X-ray scattering: a bridge between RNA secondary structures and three-dimensional topological structures.Current opinion in structural biology · 2015Review
- Methods for SAXS-based structure determination of biomolecular complexes.Advanced materials (Deerfield Beach, Fla.) · 2014Article
- Progress and outlook in structural biology of large viral RNAs.Virus research · 2014Review
- Advances in the determination of nucleic acid conformational ensembles.Annual review of physical chemistry · 2014Review
- Wide-angle X-ray solution scattering for protein-ligand binding: multivariate curve resolution with Bayesian confidence intervals.Biophysical journal · 2013Article
- Database proton NMR chemical shifts for RNA signal assignment and validation.Journal of biomolecular NMR · 2013Article
- Nucleic acid structure characterization by small angle X-ray scattering (SAXS).Current protocols in nucleic acid chemistry · 2012Article
- Imino hydrogen positions in nucleic acids from density functional theory validated by NMR residual dipolar couplings.Journal of the American Chemical Society · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Among the greatest advances in biology today are the discoveries of various roles played by RNA in biological processes. However, despite significant advances in RNA structure determination using X-ray crystallography [1] and solution NMR [2-4], the number of bona fide RNA structures is very limited, in comparison with the growing number of known functional RNAs. This is because of great difficulty in growing crystals or/and obtaining phase information, and severe size constraints on structure determination by solution NMR spectroscopy. Clearly, there is an acute need for new methodologies for RNA structure determination. The prevailing approach for structure determination of RNA in solution is a "bottom-up" approach that was basically transplanted from the approach used for determining protein structures, despite vast differences in both structural features and chemical compositions between these two types of biomacromolecules. In this chapter, we describe a new method, which has been reported recently, for rapid global structure determination of RNAs using solution-based NMR spectroscopy and small-angle X-ray scattering. The method treats duplexes as major building blocks of RNA structures. By determining the global orientations of the duplexes and the overall shape, the global structure of an RNA can be constructed and further regularized using Xplor-NIH. The utility of the method was demonstrated in global structure determination of two RNAs, a 71-nt and 102-nt RNAs with an estimated backbone RMSD ∼3.0Å. The global structure opens door to high-resolution structure determination in solution.
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